FATZ, a filamin-, actinin-, and telethonin-binding protein of the Z-disc of skeletal muscle
FATZ, a filamin-, actinin-, and telethonin-binding protein of the Z-disc of skeletal muscle
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DOI：
10.1074/jbc.m007493200
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发表时间：
2000-12-29
影响因子：
4.8
通讯作者：
Lanfranchi, G
中科院分区：
文献类型：
--
作者：
Faulkner, G;Pallavicini, A;Lanfranchi, G
我们报告了一种在骨骼肌中表达并位于肌节 Z 盘中的新型 32 kDa 蛋白质的鉴定和表征。我们发现该蛋白与其他三种 Z 盘蛋白结合；因此，我们将其命名为FATZ、γ-丝蛋白/ABP-L、α-肌动蛋白和Z-盘的telethonin结合蛋白。从酵母双杂交实验中，我们能够表明，α-肌动蛋白 2 的 SR3-SR4 结构域需要与 FATZ 的 COOH 末端区域结合，就像 γ-丝蛋白/ABP-L 一样。此外，通过使用谷胱甘肽 S-转移酶重叠测定，我们发现 FATZ 也与 telethonin 结合。肌肉细胞中 FATZ 蛋白的水平在分化过程中增加，在肌球蛋白出现之前就可以清楚地检测到。尽管 FATZ 没有已知的相互作用域，但它似乎参与了与其他 Z 带成分相互作用的复杂网络。根据有关其结合伙伴的已知信息，我们可以设想 FATZ 在以下方面的核心作用：肌原纤维形成，在筛选我们的肌肉表达序列标签数据库和公共表达序列标签数据库后，我们能够组装另外两个肌肉转录本，它们在两个不同的域中与 FATZ 显示出高度的同一性。因此，FATZ 可能是新型肌肉蛋白小家族的第一个成员。
We report the identification and characterization of a novel 32-kDa protein expressed in skeletal muscle and located in the Z-disc of the sarcomere. We found that this protein binds to three other Z-disc proteins; therefore, we have-named it FATZ, gamma -filamin/ABP-L, alpha -actinin and telethonin binding protein of the Z-disc. From yeast two-hybrid experiments we are able to show that the SR3-SR4 domains of alpha -actinin 2 are required to bind the COOH-terminal region of the FATZ as does gamma -filamin/ABP-L, Furthermore, by using a glutathione S-transferase overlay assay we find that FATZ also binds telethonin. The level of FATZ protein in muscle cells increases during differentiation, being clearly detectable before the onset of myosin, Although FATZ has no known interaction domains, it would appear to be involved in a complex network of interactions with other Z-band components. On the basis of the information known about its binding partners, we could envisage a central role for FATZ in the: myofibrillogenesis, After screening our muscle expressed sequence tag data base and the public expressed sequence tag data bases, we were able to assemble two other muscle transcripts that show a high level of identity with FATZ in two different domains. Therefore, FATZ may be the first member of a small family of novel muscle proteins.