In situ replication of immediate dendritic cell (DC) precursors contributes to conventional DC homeostasis in lymphoid tissue
In situ replication of immediate dendritic cell (DC) precursors contributes to conventional DC homeostasis in lymphoid tissue
复制标题
DOI：
10.4049/jimmunol.176.12.7196
复制
发表时间：
2006-06-15
影响因子：
4.4
通讯作者：
Cattral, Mark S.
中科院分区：
文献类型：
--
作者：
Diao, Jun;Winter, Erin;Cattral, Mark S.
在生理条件下树突状细胞（DC）的发育生物学尚不清楚。在这项研究中，我们表明小鼠CD11C（+）MHC II（ - ）谱系（ - ）细胞是常规DC的直接前体，并且广泛分布在骨髓和淋巴组织中。这些前体具有较高的克隆效率，当在支撑性基质单层上共培养或在体内转移时，会产生人群CD11C（+）MHC II类+ DC，以保留有限的增殖能力。在稳态条件下，一小部分的直接直流前体（DCP）和DC在骨髓和脾脏中积极分裂。定义了启动和支持立即DCP增殖的细胞因子。总的来说，我们的发现提供了骨髓和淋巴组织中常规DC的独特发展途径的证据，并强调了立即复制DCP和DC在维持常规DC种群中的重要性。
The developmental biology of dendritic cells (DC) under physiological conditions remains unclear. In this study, we show that mouse CD11c(+) MHC class II(-)lineage(-) cells are immediate precursors of conventional DC and are widely distributed in both bone marrow and lymphoid tissues. These precursors have a high clonal efficiency, and when cocultured on a supportive stromal monolayer or adoptively transferred in vivo, generate a population CD11c(+)MHC class II+ DC that retain limited proliferation capacity. During steady state conditions, a small proportion of immediate DC precursors (DCp) and DCs are dividing actively in bone marrow and spleen. Cytokines that initiate and support proliferation of immediate DCp were defined. Collectively, our findings provide evidence of a distinct development pathway for conventional DC in both bone marrow and lymphoid tissues and highlight the importance of in situ replication of immediate DCp and DC in maintaining conventional DC populations.