Protective effect of pretreatment with the calcium antagonist anipamil on the ischemic-reperfused rat myocardium: a phosphorus-31 nuclear magnetic resonance study.
Protective effect of pretreatment with the calcium antagonist anipamil on the ischemic-reperfused rat myocardium: a phosphorus-31 nuclear magnetic resonance study.
复制标题
钙拮抗剂阿尼帕米预处理对缺血再灌注大鼠心肌的保护作用：磷 31 核磁共振研究。
DOI：
10.1016/s0735-1097(98)90069-9
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发表时间：
1988
影响因子：
24
通讯作者：
F. Meijler
中科院分区：
文献类型：
--
作者：
J. Kirkels;T. Ruigrok;C. V. van Echteld;F. Meijler
为了评估预防性给予阿尼帕米（一种新的钙拮抗剂）是否保护心脏免受缺血和再灌注的影响，每天两次向大鼠腹腔内注射5 mg/kg体重的这种药物，持续5天。然后通过Langendorff技术分离和灌注心脏。用~（31）P核磁共振波谱法监测对照灌注和30 min完全缺血（37 °C）及随后30 min再灌注期间心肌能量代谢和细胞内pH值，阿尼帕米预处理既不改变常氧条件下左心室发展压，也不改变缺血期间三磷酸腺苷（ATP）和磷酸肌酸消耗的速率和程度。细胞内酸化，然而，衰减。再灌注时，从阿尼帕米预处理的动物的心脏恢复显着优于未经处理的心脏方面的ATP和磷酸肌酸商店的补充，恢复低水平的细胞内无机磷酸盐和左心室功能和冠状动脉流量的恢复。细胞内的pH值迅速恢复到缺血前的水平，而在未经处理的心脏一个复杂的细胞内无机磷酸盐的峰值表明存在不同的pH值的区域内myocardial.It的结论是阿尼帕米预处理保护心脏对缺血和再灌注的一些有害影响。由于这种保护作用发生在无负性肌力作用的情况下，在常氧，它不能归因于缺血期间的能量节约效应。因此，应考虑替代作用机制。
To assess whether the prophylactic administration of anipamil, a new calcium antagonist, protects the heart against the effects of ischemia and reperfusion, rats were injected intraperitoneally twice daily for 5 days with 5 mg/kg body weight of this drug. The heart was then isolated and perfused by the Langendorff technique. Phosphorus-31 nuclear magnetic resonance spectroscopy was used to monitor myocardial energy metabolism and intracellular pH during control perfusion and 30 min of total ischemia (37 °C), followed by 30 min of reperfusion.Pretreatment with anipamil altered neither left ventricular developed pressure under normoxic conditions nor the rate and extent of depletion of adenosine triphosphate (ATP) and creatine phosphate during ischemia. Intracellular acidification, however, was attenuated.On reperfusion, hearts from anipamil-pretreated animals recovered significantly better than untreated hearts with respect to replenishment of ATP and creatine phosphate stores, restitution of low levels of intracellular inorganic phosphate and recovery of left ventricular function and coronary flow. Intracellular pH recovered rapidly to preischemic levels, whereas in untreated hearts a complex intracellular inorganic phosphate peak indicated the existence of areas of different pH within the myocardium.It is concluded that anipamil pretreatment protects the heart against some of the deleterious effects of ischemia and reperfusion. Because this protection occurred in the absence of a negative inotropic effect during normoxia, it cannot be attributed to an energy-sparing effect during ischemia. Therefore, alternative mechanisms of action are to be considered.
通过表面线圈磷 31 NMR 评估心肌梗死的药物治疗。
DOI：
10.1126/science.7444460
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Nunnally,RL;Bottomley,PA
通讯作者：
Bottomley,PA