An Antiinflammatory and Reactive Oxygen Species Suppressive Effects of an Extract of Polygonum Cuspidatum Containing Resveratrol
An Antiinflammatory and Reactive Oxygen Species Suppressive Effects of an Extract of Polygonum Cuspidatum Containing Resveratrol
复制标题
DOI：
10.1210/jc.2010-0482
复制
发表时间：
2010-09-01
影响因子：
5.8
通讯作者：
Dandona, Paresh
中科院分区：
文献类型：
--
作者：
Ghanim, Husam;Sia, Chang Ling;Dandona, Paresh
背景：白藜芦醇在体外和动物模型中已被证明具有抗炎和抗衰老作用。 目的：本研究的目的是研究含有白藜芦醇的虎杖提取物 (PCE) 对正常受试者氧化和炎症应激的影响。 研究设计和方法： 两组（每组 10 名）正常体重的健康受试者被随机分配到安慰剂组或含有 40 毫克的 PCE 组。 每天毫克白藜芦醇，持续 6 周。在治疗前和治疗后第 1、3 和 6 周采集空腹血样。单核细胞用于活性氧生成、RNA 分离、核提取和总细胞匀浆制备。通过 RT-PCR 和蛋白质印迹法测量氧化和炎症应激指数、细胞因子信号传导抑制剂 3、磷酸酪氨酸磷酸酶 1B、jun-N 末端激酶 1 和 kappa B-激酶-β 抑制剂。结果：提取物显着减少活性氧生成、p47(phox) 表达、核内核 与基线和安慰剂相比，单核细胞中因子-κ B 结合、jun-N 末端激酶-1、κ B-激酶-β 抑制剂、磷酸酪氨酸磷酸酶-1B 和细胞因子信号传导-3 抑制剂的表达。 PCE 摄入量还抑制了 TNF-α、IL-6 和 C 反应蛋白的血浆浓度。给予安慰剂的对照组这些指标没有变化。结论：含PCE的白藜芦醇对氧化和炎症应激具有全面的抑制作用。 （临床内分泌代谢杂志 95：E1-E8，2010）
Background: Resveratrol have been shown to exert an antiinflammatory and antiaging effects in vitro and in animal models.Objective: The objective of the study was to investigate the effect of a Polygonum cuspidatum extract (PCE) containing resveratrol on oxidative and inflammatory stress in normal subjects.Research Design and Methods: Two groups (10 each) of normal-weight healthy subjects were randomized to placebo or PCE containing 40 mg resveratrol daily for 6 wk. Fasting blood samples were obtained prior to and after treatment at 1, 3, and 6 wk. Mononuclear cells were prepared for reactive oxygen species generation, RNA isolation, nuclear extract, and total cell homogenate preparation. Indices of oxidative and inflammatory stress, suppressor of cytokine signaling-3, phosphotyrosine phosphatase-1B, jun-N-terminal kinase-1, and inhibitor of kappa B-kinase-beta were measured by RT-PCR and Western blotting.Results: The extract induced a significant reduction in reactive oxygen species generation, the expression of p47(phox), intranuclear nuclear factor-kappa B binding, and the expression of jun-N-terminal kinase-1, inhibitor of kappa B-kinase-beta, phosphotyrosine phosphatase-1B, and suppressor of cytokine signaling-3 in mononuclear cells when compared with the baseline and the placebo. PCE intake also suppressed plasma concentrations of TNF-alpha, IL-6, and C-reactive protein. There was no change in these indices in the control group given placebo.Conclusions: The PCE-containing resveratrol has a comprehensive suppressive effect on oxidative and inflammatory stress. (J Clin Endocrinol Metab 95: E1-E8, 2010)