A novel fabrication process of up-scalable microfiber-shaped tendon-like tissue with high cell density for uniformed macroscale assembly
A novel fabrication process of up-scalable microfiber-shaped tendon-like tissue with high cell density for uniformed macroscale assembly
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DOI：
10.1002/bit.28039
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发表时间：
2022-02-02
影响因子：
3.8
通讯作者：
Onoe, Hiroaki
中科院分区：
文献类型：
--
作者：
Fukada, Keisuke;Tachibana, Koji;Onoe, Hiroaki
本文描述了用于体外宏观肌腱组织重建的可扩展的微纤维状组织。 C3H10T1/2 细胞被封装在通过双同轴微流体装置制造的海藻酸钙水凝胶微纤维中。 C3H10T1/2细胞逐渐融合，构建微纤维状腱样组织。我们的微纤维状肌腱样组织是活的，并在 600 天的时间内保持其微纤维状形态。免疫染色和实时定量聚合酶链反应分析表明，我们制造的微纤维状肌腱样组织正确表达了肌腱调节蛋白和肌动蛋白丝的方向，这是体内肌腱组织的特征之一。此外，通过将微纤维状肌腱样组织捆绑在一起，成功构建了长度约为1厘米、厚度约为200微米的宏观肌腱组织组件。这一特征使我们能够制造具有均匀细胞分布的宏观肌腱组织。我们相信，我们制造的微纤维状肌腱样组织将是体外重建肌腱组织以治疗肌腱相关损伤的合适策略。
This paper describes up-scalable microfiber-shaped tissues for macroscale tendon tissue reconstruction in vitro. C3H10T1/2 cells were encapsulated in a calcium alginate hydrogel microfiber that was fabricated via a double coaxial microfluidic device. The C3H10T1/2 cells gradually merged to construct the microfiber-shaped tendon-like tissue. Our microfiber-shaped tendon-like tissues were alive and maintained their microfiber-shaped morphology over 600 days. Immunostaining and real-time quantitative polymerase chain reaction analyses showed that our fabricated microfiber-shaped tendon-like tissue properly expressed tenomodulin and the orientation of the filaments of actin, which are one of the characteristics of tendon tissue in vivo. Furthermore, a macroscale tendon tissue assembly with similar to 1 cm in length and similar to 200 mu m in thickness was successfully constructed by bundling the microfiber-shaped tendon-like tissues together. This feature enabled us to fabricate a macroscale tendon tissue with uniform cell distribution. We believe that our fabricated microfiber-shaped tendon-like tissue would be a suitable strategy to reconstruct tendon tissue in vitro for the treatments of tendon-related injuries.