Amplification of a serine esterase gene is involved in insecticide resistance in Sri Lankan Culex tritaeniorhynchus
Amplification of a serine esterase gene is involved in insecticide resistance in Sri Lankan Culex tritaeniorhynchus
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丝氨酸酯酶基因的扩增与斯里兰卡三带喙库蚊的杀虫剂抗性有关
DOI：
10.1046/j.1365-2583.1998.740307.x
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发表时间：
1998
影响因子：
2.6
通讯作者：
J. Hemingway
中科院分区：
文献类型：
--
作者：
S. Karunaratne;A. Vaughan;M. Paton;J. Hemingway
三带喙库蚊（Culex tritaeniorhynchus）是斯里兰卡流行性乙型脑炎的主要媒介，对有机磷杀虫剂具有抗药性，在毒死蜱的LC 50时，抗药性比率为10倍，并且在昆虫田间种群中具有高度异质性。该物种的主要抗性机制，如蚊子C。quinquefasciatus，是酯酶活性升高。基本的生化、免疫学和分子生物学分析表明，C。三带喙线虫CtrEstβ1基因与C.致倦犬扩增Est 7 β; Estβ21抗血清与CtrEstβ11发生强烈交叉反应。其相应的cDNA序列与C.致倦草该基因在C.三带喙龙同一酯酶基因在两个独立的物种中扩增，沿着在C. quinquefasciatus表明该基因在基因组中的位置使其易于扩增。
Culex tritaeniorhynchus, the major vector of Japanese encephalitis in Sri Lanka, is resistant to organophosphorus insecticides, with a 10‐fold resistance ratio at the LC50 for chlorpyrifos, and a high heterogeneity factor in the insect field population. The major mechanism of resistance in this species, as in the mosquito C. quinquefasciatus, is elevation of esterase activity. Basic biochemical, immunological and molecular analysis suggests that the C. tritaeniorhynchus CtrEstβ1 gene is orthologous to the C. quinquefasciatus amplified Est7beta;s. The Estβ21 antiserum cross‐reacts strongly with CtrEstβ11. Its corresponding cDNA, over the 545 base pairs sequenced, has ∼84% identity with the various C. quinquefasciatus Estβs. The gene is amplified in C. tritaeniorhynchus. Amplification of the same esterase in two independent species, along with multiple amplification events involving this esterase gene in C. quinquefasciatus suggests that the location of this gene within the genome predisposes it to amplification.