Reconstitution of an Argonaute-dependent small RNA biogenesis pathway reveals a handover mechanism involving the RNA exosome and the exonuclease QIP.
Reconstitution of an Argonaute-dependent small RNA biogenesis pathway reveals a handover mechanism involving the RNA exosome and the exonuclease QIP.
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DOI：
10.1016/j.molcel.2012.03.019
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发表时间：
2012-05-11
期刊：
影响因子：
16
通讯作者：
Liu, Yi
中科院分区：
文献类型：
--
作者：
Xue, Zhihong;Yuan, Haiyan;Guo, Jinhu;Liu, Yi
Argonaute proteins are required for the biogenesis of some small RNAs (sRNAs), including the PIWI-interacting RNAs and some microRNAs. How Argonautes mediate maturation of sRNAs independent of their slicer activity is not clear. The maturation of the Neurospora miRNA-like sRNA, milR-1, requires the Argonaute protein QDE-2, Dicer, and QIP. Here, we reconstitute this Argonaute-dependent sRNA biogenesis pathway in vitro and discover that the RNA exosome is also required for milR-1 production. Our results demonstrate that QDE-2 mediates milR-1 maturation by recruiting exosome and QIP and by determining the size of milR-1. The exonuclease QIP first separates the QDE-2-bound pre-milR-1 duplex and then mediates 3’ to 5’ trimming and maturation of pre-milRNA together with exosome using a hand-over mechanism. In addition, exosome is also important for the decay of sRNAs. Together, our results establish a biochemical mechanism of an Argonaute-dependent sRNA biogenesis pathway and critical roles of exosome in sRNA processing.
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影响因子：
64.5
作者：
Halic M;Moazed D
通讯作者：
Moazed D
影响因子：
10.5
作者：
Cheng, P;He, Q;Liu, Y
通讯作者：
Liu, Y
影响因子：
16.8
作者：
Haley, B;Zamore, PD
通讯作者：
Zamore, PD
影响因子：
16.8
作者：
Dziembowski, Andrzej;Lorentzen, Esben;Seraphin, Bertrand
通讯作者：
Seraphin, Bertrand
DOI：
10.1126/science.1190809
发表时间：
2010-06-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Cifuentes D;Xue H;Taylor DW;Patnode H;Mishima Y;Cheloufi S;Ma E;Mane S;Hannon GJ;Lawson ND;Wolfe SA;Giraldez AJ
通讯作者：
Giraldez AJ