Immune system: maturation of myeloid cells.
Immune system: maturation of myeloid cells.
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DOI：
10.1007/978-1-60327-009-0_12
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Girard, Denis
中科院分区：
文献类型：
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作者：
Ennaciri, Jamila;Girard, Denis
人类和动物细胞系是重要的实验室工具，可用于研究各种细胞功能。一些细胞系在成熟的某个步骤被阻断，可用于研究细胞成熟的机制。由于此类细胞系在添加试剂后可以分化为更成熟的表型，因此它们可以在大型筛选实验中替代原代细胞，从而避免日常分离。人类早幼粒细胞 HL-60 白血病细胞系是研究骨髓细胞中发生的事件的一个有吸引力的模型。有趣的是，这些细胞可以分化为单核细胞、巨噬细胞或粒细胞，如本章所述。
Human and animal cell lines are important laboratory tools that can be used for studying a variety of cell functions. Some cell lines are blocked at a certain step of maturation and can be used in order to study the mechanisms involved in cell maturation. Because such cell lines can be differentiated toward a more mature-like phenotype after the addition of agents, they can replace primary cells in large screening experiments preventing daily isolation. The human promyelocytic HL-60 leukemia cell line is an attractive model for studying the events which occur in myeloid cells. Interestingly, these cells can be differentiated toward monocytes, macrophages, or granulocytes as described in the present chapter.