Ethanol fractionation precipitation and physico-chemical properties of soluble polysaccharides from Prunella vulgaris Linn
Ethanol fractionation precipitation and physico-chemical properties of soluble polysaccharides from Prunella vulgaris Linn
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发表时间：
2012
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通讯作者：
Nie Shao-ping
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作者：
Nie Shao-ping
对夏枯草水溶性多糖的理化性质进行了研究.采用热水浸提、乙醇沉淀的方法提取可溶性多糖，经20%、30%、40%、50%、60%和70%乙醇梯度洗脱，得到6个组分，分别命名为PVLP-1、PVLP-2、PVLP-3、PVLP-4、PVLP-5和PVLP-6。测定了各组分中糖、糖醛酸和蛋白质的含量，并采用气相色谱法分析了各组分的单糖组成，同时进行了紫外光谱和红外光谱分析，以确定其光谱性质。结果表明，6个组分均为酸性多糖，蛋白质含量较高。各组分均含有鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖，其中木糖和阿拉伯糖是主要单糖，甘露糖含量最低，未发现岩藻糖和核糖，但各组分单糖的摩尔比不同。
The physico -chemical properties of soluble polysaccharides from Prunella Vulgaris Linn were studied. Soluble polysaccharides extracted with hot water and subsequently precipitated with ethanol,were successively fractionated by different gradient concentrations of ethanol (20%,30%,40%,50%,60% and 70%) into six fractions,named as PVLP -1,PVLP -2,PVLP -3,PVLP -4,PVLP -5 and PVLP -6. Contents of sugar, uronic acid and protein in these fractions were determined , monosaccharide compositions of them were also analyzed by gas chromatography(GC),UV and IR spectra analysis were run simultaneously to determine their spectroscopy properties . The result showed that all six fractions were acidic polysaccharides , with high protein content . Each of these fractions all contain rhamnose , arabinose , xylose , mannose , glucose and galactose,and xylose and arabinose were the main monosaccharides in them ,the content of mannose was lowest,fucose and ribose were not found ,but the mol ratios of these monosaccharides in six fractions were different.