Porcine Dermis and Pericardium-Based, Non-Cross-Linked Materials Induce Multinucleated Giant Cells After Their In Vivo Implantation: A Physiological Reaction?
Porcine Dermis and Pericardium-Based, Non-Cross-Linked Materials Induce Multinucleated Giant Cells After Their In Vivo Implantation: A Physiological Reaction?
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DOI：
10.1563/aaid-joi-d-14-00155
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发表时间：
2015-12-01
影响因子：
1.6
通讯作者：
Ghanaati, Shahram
中科院分区：
文献类型：
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作者：
Barbeck, Mike;Lorenz, Jonas;Ghanaati, Shahram
本研究分析了两种新型猪源性胶原材料的组织反应：心包和真皮。采用小鼠皮下植入模型，分别在植入后3、10、15、30、60 d 5个时间点观察组织反应。采用组织学、组织化学、免疫组织学和组织形态计量学分析方法。真皮来源的材料经历了早期降解，同时诱导单核细胞和一些多核巨细胞和轻度血管形成。心包源性膜诱导2种不同的细胞组织反应。致密表面诱导单核细胞和多核巨细胞，并在第30天完全降解。膜的海绵状表面主要诱导单核细胞，并作为一个稳定的屏障膜长达60天。海绵状物质表层未见跨膜血管形成。目前的数据表明，细胞组织对来自不同组织的胶原基材料的反应存在差异。此外，多核巨细胞的存在显然与物质分解/降解和血管化有关。需要进一步的临床数据来评估这里观察到的多核巨细胞的存在对人体组织内材料稳定性、整合以及相应的组织再生的影响程度。
The present study analyzed the tissue reaction to 2 novel porcine-derived collagen materials: pericardium versus dermis. By means of the subcutaneous implantation model in mice, the tissue reactions were investigated at 5 time points: 3, 10, 15, 30, and 60 days after implantation. Histologic, histochemical, immunhistologic, and histomorphometric analysis methodologies were applied. The dermis-derived material underwent an early degradation while inducing mononuclear cells together with some multinucleated giant cells and mild vascularization. The pericardium-derived membrane induced 2 different cellular tissue reactions. The compact surface induced mononuclear cells and multinucleated giant cells, and underwent a complete degradation until day 30. The spongy surface of the membrane induced mainly mononuclear cells, and served as a stable barrier membrane for up to 60 days. No transmembranous vascularization was observed within the spongy material surface layer. The present data demonstrate the diversity of the cellular tissue reaction toward collagen-based materials from different tissues. Furthermore, it became obvious that the presence of multinucleated giant cells was associated with the material breakdown/degradation and vascularization. Further clinical data are necessary to assess extent to which the presence of multinucleated giant cells observed here will influence the materials stability, integration, and, correspondingly, tissue regeneration within human tissue.