Phylogeny and divergence of basal angiosperms inferred from APETALA3- and PISTILLATA-like MADS-box genes
Phylogeny and divergence of basal angiosperms inferred from APETALA3- and PISTILLATA-like MADS-box genes
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DOI：
10.1007/s10265-004-0153-7
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发表时间：
2004-06-01
影响因子：
2.8
通讯作者：
Ito, M
中科院分区：
文献类型：
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作者：
Aoki, S;Uehara, K;Ito, M
由aptala3 （AP3）和pisttillata （PI）组成的b类MADS-box基因在开花植物的花瓣和雄蕊鉴定中起着重要作用。研究了5个基部被子植物科8种植物ap3样和pi样MADS-box基因的多样性：卡罗来纳州的布氏菌和卡龙巴（卡龙巴科）；芡实、芡实和龙花（龙花科）；茴香八头莲（八头莲科）；和五味子（五味子科）。序列分析结果显示，在先前报道的被子植物PI基因中，有一个K结构域缺失4个氨基酸，而在五仙科PI同源基因中，有6个PI同源基因缺失，这表明在被子植物中，amborellacae、cabombacae和nyphae科PI同源基因中都没有缺失，这表明amborellacae、cabombacae和nyphae科PI同源基因中都没有缺失。分子系统发育分析的结果与这一假设并不矛盾。Amborella的AP3和PI同源物在第7外显子5'区共享5个氨基酸序列。利用线性化树和似然方法，估计AP3和PI谱系之间的分化时间介于被子植物和现存裸子植物分裂后的立即到几千万年之间。所有现存被子植物最近的共同祖先的年龄估计在140-210 Ma之间，这取决于所使用的树木和所做的假设。
The B-class MADS-box genes composed of APETALA3 (AP3) and PISTILLATA (PI) lineages play an important role in petal and stamen identity in previously studied flowering plants. We investigated the diversification of the AP3-like and PI-like MADS-box genes of eight species in five basal angiosperm families: Amborella trichopoda (Amborellaceae); Brasenia schreberi and Cabomba caroliniana (Cabombaceae); Euryale ferox, Nuphar japonicum, and Nymphaea tetragona (Nymphaeaceae); Illicium anisatum (Illiciaceae); and Kadsura japonica (Schisandraceae). Sequence analysis showed that a four amino acid deletion in the K domain, which was found in all previously reported angiosperm PI genes, exists in a PI homologue of Schisandraceae, but not in six PI homologues of the Amborellaceae, Cabombaceae, and Nymphaeaceae, suggesting that the Amborellaceae, Cabombaceae, and Nymphaeaceae are basalmost lineages in angiosperms. The results of molecular phylogenetic analyses were not inconsistent with this hypothesis. The AP3 and PI homologues from Amborella share a sequence of five amino acids in the 5' region of exon 7. Using the linearized tree and likelihood methods, the divergence time between the AP3 and PI lineages was estimated as somewhere between immediately after to several tens of millions of years after the split between angiosperms and extant gymnosperms. Estimates of the age of the most recent common ancestor of all extant angiosperms range from similar to140-210 Ma, depending on the trees used and assumptions made.