Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens.
Isoelectric focusing and reverse immunoblotting of autoantibodies against high molecular weight antigens.
复制标题
针对高分子量抗原的自身抗体的等电聚焦和反向免疫印迹。
DOI：
--
复制
发表时间：
1986
影响因子：
2.8
通讯作者：
J. Mclearie
中科院分区：
文献类型：
--
作者：
D. Stott;J. Mclearie
高M.Wt.抗体克隆分析方法对抗原进行了描述。它包括等电聚焦、将聚焦的抗体电泳化转移到硝酸纤维素膜，以及通过覆盖放射性标记的抗原来检测膜结合的抗体(反向免疫印迹)。描述了该技术在抗甲状腺球蛋白和DNA自身抗体的克隆分析中的应用。
A method for the clonal analysis of antibodies against high M.Wt. antigens is described. It involves isoelectric focusing, electrophoretic transfer of the focused antibodies to a nitrocellulose membrane and detection of the membrane-bound antibodies by overlay with radiolabelled antigen (reverse immunoblotting). The application of this technique to the clonal analysis of autoantibodies against thyroglobulin and DNA is described.