Clathrin-Mediated Endocytosis of Gold Nanoparticles In Vitro
Clathrin-Mediated Endocytosis of Gold Nanoparticles In Vitro
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DOI：
10.1002/ar.23051
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发表时间：
2015-02-01
影响因子：
2
通讯作者：
Bay, Boon Huat
中科院分区：
文献类型：
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作者：
Cheng Teng Ng;Tang, Florence Mei Ai;Bay, Boon Huat
金纳米粒子（AuNPs）具有潜在的生物医学和科学应用。在这项研究中，我们评估了不同的显微镜技术的吸收和内化的FBS涂层的20纳米金纳米粒子到肺成纤维细胞和肝细胞。在光镜下观察到MRC 5肺成纤维细胞和Chang肝细胞内的AuNP聚集体，尤其是在用自描记术增强后。通过扫描电子显微镜观察到金纳米粒子簇被吸附在细胞表面上。超薄切片显示，金纳米粒子主要封闭在细胞质囊泡时，在透射电镜下观察。我们还研究了金纳米颗粒的吸收机制，使用内吞抑制剂和定量的Au与电感耦合等离子体质谱。用伴刀豆球蛋白A和氯丙嗪处理的细胞分别在MRC 5肺成纤维细胞和Chang肝细胞中显示出Au摄取的显著降低，这意味着AuNPs的摄取通过网格蛋白介导的内吞作用而促进。因此，似乎在具有不同来源组织的两种细胞类型中20 nm AuNP的摄取依赖于网格蛋白介导的内吞作用。Anat Rec，298：418-427，2015. (c)2014 Wiley Periodicals，Inc.
Gold nanoparticles (AuNPs) have potential biomedical and scientific applications. In this study, we evaluated the uptake and internalization of FBS-coated 20 nm AuNPs into lung fibroblasts and liver cells by different microscopy techniques. AuNP aggregates were observed inside MRC5 lung fibroblasts and Chang liver cells under light microscopy, especially after enhancement with automegallography. Clusters of AuNPs were observed to be adsorbed on the cell surface by scanning electron microscopy. Ultrathin sections showed that AuNPs were mainly enclosed within cytoplasmic vesicles when viewed under transmission electron microscopy. We also investigated the mechanism of uptake for AuNPs, using endocytosis inhibitors and quantification of Au with inductively coupled plasma mass spectrometry. Cells treated with concanavalin A and chlorpromazine showed significant decrease of Au uptake in MRC5 lung fibroblasts and Chang liver cells, respectively, implying that the uptake of AuNPs was facilitated by clathrin-mediated endocytosis. It would therefore appear that uptake of 20 nm AuNPs in both cell types with different tissues of origin, was dependent upon clathrin-mediated endocytosis. Anat Rec, 298:418-427, 2015. (c) 2014 Wiley Periodicals, Inc.