DIFFERENTIATION OF LISTERIA-MONOCYTOGENES AND LISTERIA-INNOCUA BY 16S RIBOSOMAL-RNA GENES AND INTRASPECIES DISCRIMINATION OF LISTERIA-MONOCYTOGENES STRAINS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMORPHISMS
DIFFERENTIATION OF LISTERIA-MONOCYTOGENES AND LISTERIA-INNOCUA BY 16S RIBOSOMAL-RNA GENES AND INTRASPECIES DISCRIMINATION OF LISTERIA-MONOCYTOGENES STRAINS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMORPHISMS
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DOI：
10.1128/aem.59.1.304-308.1993
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发表时间：
1993-01-01
影响因子：
4.4
通讯作者：
BATT, CA
中科院分区：
文献类型：
--
作者：
CZAJKA, J;BSAT, N;BATT, CA
已检测到可用于区分单核细胞增生李斯特菌与无害李斯特菌的16 S rRNA基因（16 S rDNA）的差异。16 S rDNA通过聚合酶链反应与一组寡核苷酸引物侧翼的1.5-kb片段扩增。在16 S rDNA的V2区，两种L.单核细胞增多症Scott A和L. innocua和不同L.单核细胞增多症血清型。虽然L.单核细胞增多症SLCC 2371与L. innocua，这两个物种在核苷酸1259和1292处的V9区域内是不同的，与先前的研究（R. F.王维W. Cao和M. G.约翰逊，应用环境。Microbiol. 57：3666-3670，1991）。L.的种内鉴别。单核细胞增多症菌株通过使用随机扩增多态性DNA引物产生的模式来实现。虽然在L中可以进行一些区分。虽然单核细胞增多症物种通过它们的16 S rDNA序列来区分，但是可以通过从染色体DNA产生随机扩增多态性DNA模式来在物种内进行更大的区分。通过使用一些10 bp的引物，在所有的情况下，每个分离物的独特模式，在不同的L。可以产生单核细胞增多症血清型，即使它们可能具有相同的16 S rRNA序列。
Differences in the 16S rRNA genes (16S rDNA) which can be used to discriminate Listeria monocytogenes from Listeria innocua have been detected. The 16S rDNA were amplified by polymerase chain reaction with a set of oligonucleotide primers which flank a 1.5-kb fragment. Sequence differences were observed in the V2 region of the 16S rDNA both between L. monocytogenes Scott A and L. innocua and between different L. monocytogenes serotypes. Although L. monocytogenes SLCC2371 had the same V2 region sequence as L. innocua, the two species were different within the V9 region at nucleotides 1259 and 1292, in agreement with previous studies (R.-F. Wang, W.-W. Cao, and M. G. Johnson, Appl. Environ. Microbiol. 57:3666-3670, 1991). Intraspecies discrimination of L. monocytogenes strains was achieved by using the patterns generated by random amplified polymorphic DNA primers. Although some distinction can be made within the L. monocytogenes species by their 16S rDNA sequence, a far greater discrimination within species could be made by generating random amplified polymorphic DNA patterns from chromosomal DNA. By using a number of 10-bp primers, unique patterns for each isolate which in all cases examined differentiate between various L. monocytogenes serotypes, even though they may have the same 16S rRNA sequences, could be generated.