Implications for tooth eruption of the effect of interleukin-1α on nuclear factor-κB gene expression in the rat dental follicle
Implications for tooth eruption of the effect of interleukin-1α on nuclear factor-κB gene expression in the rat dental follicle
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DOI：
10.1016/s0003-9969(99)00071-0
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发表时间：
1999-11-01
影响因子：
3
通讯作者：
Wise, GE
中科院分区：
文献类型：
--
作者：
Que, BG;Lumpkin, SJ;Wise, GE
牙萌出是一种局部性事件，在牙囊和星状网中产生的一系列分子信号似乎启动了牙萌出。因此，单个核细胞被招募到毛囊中，然后融合成为破骨细胞，需要吸收牙槽骨形成喷发途径。参与分子信号转导序列的转录因子之一可能是核因子kappaB。本研究表明，核因子kappaB是由培养的牙囊细胞表达和合成的。此外，它的转录、激活和转位也被潜在的出疹分子白介素1α所增强。IL-1α对核因子kappa B基因转录的增强作用可被酪氨酸特异性的激酶抑制剂阻断，提示这种增强可能需要核因子kappa B复合体的磷酸化。在体内，核因子kappaB在出生后3d的大鼠下颌第一磨牙的牙囊中表达最高，这一年龄是单个核细胞流入毛囊的高峰期。因此，一种显然是出疹所需的转录因子(核因子kappaB)存在于出疹所需的组织中，即牙囊，其基因表达在出疹的关键时刻达到最大。(C)1999爱思唯尔科学有限公司。保留所有权利。
Tooth eruption is a localized event and a cascade of molecular signals generated in the dental follicle and stellate reticulum appears to initiate its onset. Consequently, mononuclear cells are recruited into the follicle and, in turn, fuse to become osteoclasts needed to resorb the alveolar bone to form an eruption pathway. One of the transcription factors involved in the sequence of molecular signalling may be nuclear factor (NF)kappa B. This study shows that NF kappa B is expressed and synthesized by cultured dental follicle cells. Moreover, its transcription, activation and translocation were enhanced by interleukin (IL)-1 alpha, a potential eruption molecule. The enhancement of transcription of the NF kappa B gene by IL-1 alpha was blocked by a tyrosine-specific kinase inhibitor, suggesting that the enhancement may require the phosphorylation of the NF kappa B complex. In vivo, NF kappa B is maximally expressed in the dental follicle of the rat first mandibular molar at day 3 postnatally, the age at which there is a peak influx of mononuclear cells into the follicle. Thus, a transcription factor apparently required for eruption (NF kappa B) is present in the tissue required for eruption, the dental follicle, and its gene expression is maximal at a critical time in eruption. (C) 1999 Elsevier Science Ltd. All rights reserved.