Arsenic bioaccessibility upon gastrointestinal digestion is highly determined by its speciation and lipid-bile salt interactions
Arsenic bioaccessibility upon gastrointestinal digestion is highly determined by its speciation and lipid-bile salt interactions
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DOI：
10.1080/10934529.2013.732367
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发表时间：
2013-05-01
影响因子：
2.1
通讯作者：
Van de Wiele, Tom R.
中科院分区：
文献类型：
--
作者：
Alava, Pradeep;Du Laing, Gijs;Van de Wiele, Tom R.
在胃肠道消化过程中，受污染的食物基质中砷(As)的释放，即其生物可获得性，是评估其生物有效性的重要指标，因此也是其健康风险的重要评估指标。此外，由于毒性主要由其形态决定，尚不清楚不同物种在上消化道消化过程中的不同表现。在这里，我们评估了胃液pH值和胆汁浓度等消化参数以及食物基质成分对8种AS的生物可获得性的影响程度(AsIII、ASV、MMAV、DMAV、MMAIII、DMAIII、MMMTAV、DMMTAV)。在pH值为1.8的条件下，所有AS标准的生物可获得性在85%到90%之间。随着胃液pH值的升高，甲基化和硫代化砷化合物的生物可利用性从85%下降到50%，而胆盐浓度增加到30g/L时，由于与胆盐中存在的铁相互作用，无机物种的生物可利用性从83%下降到70%。关于食物基质，我们注意到纤维含量不影响生物可及性，但脂肪的存在导致无机和有机砷在胆盐存在下的生物可及性增加。至于无机砷，肠道中三价铁的存在似乎是影响IAS生物可及性的主要因素。这些数据表明，必须在摄取和胃肠道消化时考虑物种依赖的生物可获得性。
The release of arsenic (As) from a contaminated food matrix during gastrointestinal digestion, i.e., its bioaccessibility, is an important estimator of its bioavailability, and therefore also its health risk. In addition, As toxicity is primarily determined by its speciation and it is not clear how different As species behave during digestion in the upper digestive tract. Here, we evaluated to what extent digestive parameters like gastric pH and bile concentration, but also food matrix constituents affect the bioaccessibility of 8 As species (AsIII, AsV, MMAV, DMAV, MMAIII, DMAIII, MMMTAV, DMMTAV). Bioaccessibility of all As standards ranged between 85% and 90% under pH 1.8. Bioaccessibility of methylated and thiolated arsenicals was decreased from 85% to 50% with increasing gastric pH to 4, yet an increasing bile salts concentration up to 30g/L lowered the bioaccessibility of inorganic species from 83% to 70% due to interaction with Fe present in bile salts. With respect to food matrices, we noted that the fiber content did not affect As bioaccessibility, yet the presence of fat resulted in an increased bioaccessibility of both inorganic and organic arsenicals in the presence of bile salts. With respect to inorganic arsenic, the intestinal presence of trivalent Fe appeared to be the predominant factor for bioaccessibility of iAs. These data demonstrate that species dependent bioaccessibility must be considered upon ingestion and gastrointestinal digestion.