Chemical colloids versus biological colloids: a comparative study for the elucidation of the mechanism of protein fiber formation.
Chemical colloids versus biological colloids: a comparative study for the elucidation of the mechanism of protein fiber formation.
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化学胶体与生物胶体：阐明蛋白纤维形成机制的比较研究。
DOI：
10.1021/bi0474719
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Cabana,VeneracionG
中科院分区：
文献类型：
--
作者：
Xu,Shaohua;Wu,David;Arnsdorf,Morton;Johnson,Robert;Getz,GodfreyS;Cabana,VeneracionG
将小鼠血清淀粉样蛋白A1 （SAA）的纤维形成与胶体金颗粒的线性聚集和纤维形成进行了比较。在这里，我们报告这些过程的相似之处。经醋酸孵育后，SAA错误折叠并形成新的构象，我们称之为SAA。saa在水溶液中的可溶性明显低于saa；它聚集形成成核单位，然后形成纤维。纤维表现为成核单位的一串。此外，外加电场促进saa纤维的形成。saa的这些性质使人联想到金离子形成的胶体金和金胶体的一维聚集。胶体金颗粒也被发现能够在电场下或在聚赖氨酸的存在下一维聚集。这些金纤维在结构上与saa纤维相似。总之，蛋白质的聚集和纤维的形成似乎遵循胶体科学中原子和分子聚集的一般原理，包括聚合物如多肽。胶体金的形成和一维聚集的分析为阐明蛋白质纤维形成的某些方面提供了一个简单的模型系统。
Fiber formation from murine serum amyloid A1 (SAA) was compared to the linear aggregation and fiber formation of colloidal gold particles. Here we report the similarities of these processes. Upon incubation with acetic acid, SAA misfolds and adopts a new conformation, which we termed saa. saa apparently is less soluble than SAA in aqueous solution; it aggregates and forms nucleation units and then fibers. The fibers appear as a string of the nucleation units. Additionally, an external electric field promotes saa fiber formation. These properties of saa are reminiscent of colloidal gold formation from gold ions and one-dimensional aggregation of the gold colloids. Colloidal gold particles were also found to be capable of aggregating one-dimensionally under an electric field or in the presence of polylysine. These gold fibers resembled in structure that of saa fibers. In summary, protein aggregation and formation of fibers appear to follow the generalized principles derived in colloidal science for the aggregation of atoms and molecules, including polymers such as polypeptides. The analysis of colloidal gold formation and of one-dimensional aggregation provides a simple model system for the elucidation of some aspects of protein fiber formation.
影响因子：
6.5
作者：
Veneracion;Cabana;J. Lukens;K. Rice;Terry J. Hawkins;G. Getz
通讯作者：
Veneracion;Cabana;J. Lukens;K. Rice;Terry J. Hawkins;G. Getz
影响因子：
4.1
作者：
Patel, H;Bramall, J;Woo, P
通讯作者：
Woo, P
影响因子：
5.6
作者：
Honig, B
通讯作者：
Honig, B