ALPHA-1(XVIII), A COLLAGEN CHAIN WITH FREQUENT INTERRUPTIONS IN THE COLLAGENOUS SEQUENCE, A DISTINCT TISSUE DISTRIBUTION, AND HOMOLOGY WITH TYPE-XV COLLAGEN
ALPHA-1(XVIII), A COLLAGEN CHAIN WITH FREQUENT INTERRUPTIONS IN THE COLLAGENOUS SEQUENCE, A DISTINCT TISSUE DISTRIBUTION, AND HOMOLOGY WITH TYPE-XV COLLAGEN
复制标题
DOI：
10.1073/pnas.91.10.4234
复制
发表时间：
1994-05-10
影响因子：
11.1
通讯作者：
PIHLAJANIEMI, T
中科院分区：
文献类型：
--
作者：
REHN, M;PIHLAJANIEMI, T
我们报道了小鼠编码XVIII型胶原蛋白A1链的胶原序列的克隆的分离。重叠克隆全长2.8kb，编码928个氨基酸残基的开放阅读框，包括25个残基的信号肽、301个残基的氨基末端非胶原区和602个残基的主要胶原区。克隆不覆盖多肽的羧基末端，因为没有翻译终止密码子。所推导的多肽的特征是在胶原蛋白氨基酸序列中拥有8个长度从10到24个残基的非胶原性中断。其他特征包括N-连接的糖基化和O-连接的糖胺多糖连接的几个假定位点的存在，以及氨基末端非胶原区与凝血酶原蛋白的同源性。特别有趣的是，在8个XVIII型胶原序列中，有5个与先前报道的XVI型胶原同源，这表明这两个序列在不同的胶原蛋白家族中形成了一个不同的亚群。Northern印迹杂交分析显示，XVIII型胶原mRNAs在组织中有显著的分布，因为这些克隆与4.3kb和5.3kb的mRNAs强烈杂交，而这些mRNAs仅存在于所研究的8个小鼠组织的肺和肝脏中。
We report on the isolation of mouse cDNA clones which encode a collagenous sequence designated here as the al chain of type XVIII collagen. The overlapping clones cover 2.8 kilobases and encode an open reading frame of 928 amino acid residues comprising a putative signal peptide of 25 residues, an amino-terminal noncollagenous domain of 301 residues, and a primarily collagenous stretch of 602 residues. The clones do not cover the carboxyl-terminal end of the polypeptide, since the translation stop codon is absent. Characteristic of the deduced polypeptide is the possession of eight noncollagenous interruptions varying in length from 10 to 24 residues in the collagenous amino acid sequence. Other features include the presence of several putative sites for both N-linked glycosylation and O-linked glycosaminoglycan attachment and homology of the amino-terminal noncollagenous domain with thrombospondin. It is of particular interest that five of the eight collagenous sequences of type XVIII show homology to the previously reported type XV collagen, suggesting that the two form a distinct subgroup among the diverse family of collagens. Northern blot hybridization analysis revealed a striking tissue distribution for type XVIII collagen mRNAs, as the clones hybridized strongly with mRNAs of 4.3 and 5.3 kilobases that were present only in lung and liver of the eight mouse tissues studied.