SARS-coronavirus replicates in mononuclear cells of peripheral blood (PBMCs) from SARS patients.
SARS-coronavirus replicates in mononuclear cells of peripheral blood (PBMCs) from SARS patients.
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DOI：
10.1016/s1386-6532(03)00195-1
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发表时间：
2003-12
期刊：
影响因子：
--
通讯作者：
Dennin RH
中科院分区：
文献类型：
--
作者：
Li L;Wo J;Shao J;Zhu H;Wu N;Li M;Yao H;Hu M;Dennin RH
背景资料：严重急性呼吸综合征（SARS）的病原体是一种新近发现的单链RNA（ssRNA）阳性冠状病毒（SARS-CoV）。关于SARS病例中病毒复制型的动态变化知之甚少。目的：评估SARS-CoV是否可以感染并在受感染者的外周血单核细胞（PBMC）中复制，并揭示病毒在疾病过程中的任何动态变化。研究设计：采用逆转录-聚合酶链反应（RT-PCR）检测了同一传染源感染的SARS患者住院期间外周血单个核细胞中SARS-CoV的负链RNA（minus-RNA，"复制中间体"）和正链RNA（基因组RNA）。结果：SARS患者外周血单个核细胞中检测到SARS-CoV-RNA。PBMC中的病毒复制形式在疾病发作后6天内可检测到，而plus-RNA在更长时间内（发作后8 - 12天）可检测到。结论：SARS冠状病毒可在SARS患者外周血单个核细胞内感染和复制，但病毒在外周血单个核细胞内的复制具有自限性。
Background: The etiologic agent of severe acute respiratory syndrome (SARS) is a recently identified, positive single-stranded RNA (ssRNA) coronavirus (SARS-CoV). Little is known about the dynamic changes of the viral replicative form in SARS cases. Objectives: Evaluate whether SARS-CoV can infect and replicate in peripheral blood mononuclear cells (PBMCs) of infected persons and reveal any dynamic changes to the virus during the course of the disease. Study design: Peripheral blood mononuclear cells collected from SARS cases infected by the same infectious source were tested for both negative-stranded RNA (minus-RNA, “replicative intermediates”) and positive-stranded RNA (genomic RNA) of SARS-CoV during the course of hospitalization by reverse transcription-polymerase chain reaction (RT-PCR). Results: SARS-CoV minus-RNA was detected in PBMCs from SARS patients. The viral replicative forms in PBMCs were detectable during a period of 6 days post-onset of the disease, while the plus-RNA were detectable for a longer period (8–12 days post-onset). Conclusions: SARS-coronavirus can infect and replicate within PBMCs of SARS patients, but viral replication in PBMCs seems subject to self-limitation.
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