Simple sequence repeat (SSR) markers linked to E1, E3, E4, and E7 maturity genes in soybean
Simple sequence repeat (SSR) markers linked to E1, E3, E4, and E7 maturity genes in soybean
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作者：
Molnar, SJ;Rai, S;Cober, ER
大豆近等基因系（NILs），对比成熟度和光周期敏感性位点，基因型与约430个映射的简单序列重复（SSR），也被称为微卫星标记。通过分析近等基因系间的等位基因分布，确定了Dt1不确定生长位点的定位，完善了T黄褐色茸毛位点的SSR定位，利用分子标记定位了E1和E3成熟位点，并首次定位了E4和E7成熟位点。这些位点侧翼的分子标记现在可用于这些性状的标记辅助育种。图位分析确定了一个假定的同源关系之间的四个染色体区域，一个在中间的连锁群（LG）C2携带E1和E7，一个LG I携带E4，一个在LG C2的顶部，在那里有一个生殖期数量性状位点（QTL），和LG B1的第四个。其它证据表明，LG C2上的E1 + E7区域和LG L上与荚果成熟度QTL连锁的区域之间也存在同源性。同源性关系预测了大豆基因组中其他成熟基因座的可能位置，以及哪些成熟基因座可能具有共同的进化起源和相似的作用机制。
Soybean near isogenic lines (NILs), contrasting for maturity and photoperiod sensitivity loci, were genotyped with approximately 430 mapped simple sequence repeats (SSRs), also known as microsatellite markers. By analysis of allele distributions across the NILs, it was possible to confirm the map location of the Dt1 indeterminate growth locus, to refine the SSR mapping of the T tawny pubescence locus, to map E1 and E3 maturity loci with molecular markers, and to map the E4 and E7 maturity loci for the first time. Molecular markers flanking these loci are now available for marker-assisted breeding for these traits. Analysis of map locations identified a putative homologous relationship among four chromosomal regions; one in the middle of linkage group (LG) C2 carrying E1 and E7, one on LG I carrying E4, one at the top of LG C2, at which there is a reproductive period quantitative trait locus (QTL), and the fourth on LG B1. Other evidence suggests that homology also exists between the E1 + E7 region on LG C2 and a region on LG L linked to a pod maturity QTL. Homology relationships predict possible locations in the soybean genome of additional maturity loci, as well as which maturity loci may share a common evolutionary origin and similar mechanism(s) of action.