Denaturing gradient gel electrophoresis analysis of gut bacterial community for the Japanese earthworms
Denaturing gradient gel electrophoresis analysis of gut bacterial community for the Japanese earthworms
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DOI：
10.1080/00380768.2011.594965
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发表时间：
2011-08
影响因子：
2
通讯作者：
Kazunori Nakamura;K. Sakai
中科院分区：
文献类型：
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作者：
Kazunori Nakamura;K. Sakai
采用聚合酶链反应扩增16S rRNA基因片段（PCR-DGGE）变性梯度凝胶电泳技术，分析了日本农业草地上采集的hilgendorfi、P. heteropoda（巨型colecidae）和Allolobophora japonica （Lumbricidae）肠道细菌群落结构，并与周围土壤进行了比较。变性梯度凝胶电泳（DGGE）图谱表明，每种蚯蚓都有自己特定的细菌群落，并通过三维尺度分析将DGGE图谱分为3组：hilgendorfi和heteropoda的肠道样本、japonica的肠道样本和周围土壤的样本。通过直接测序和克隆鉴定出9个优势条带。P. hilgendorfi和P. heteropoda的主要三个条带与属于厚壁菌门的芽孢杆菌种类密切相关。粳稻的4个主要条带和2个主要条带分别与变形菌门和拟杆菌门密切相关。
The gut bacterial community structure for Pheretima hilgendorfi and P. heteropoda (Family Megascolecidae), and Allolobophora japonica (Family Lumbricidae) collected from agricultural grasslands in Japan was analyzed by denaturing gradient gel electrophoresis of polymerase chain reaction-amplified 16S rRNA gene fragments (PCR-DGGE) and compared with those in the surrounding soils. Denaturing gradient gel electrophoresis (DGGE) profiles indicated that each earthworm species had their own specific bacterial communities, and multidimentional scaling analysis grouped the DGGE profiles into three groups: gut samples from P. hilgendorfi and P. heteropoda, gut samples from A. japonica and samples from the surrounding soils. Nine dominant bands were identified by their direct sequencing and cloning. Major three bands from P. hilgendorfi and P. heteropoda were closely related to Bacillus species belonging to the phylum Firmicutes. Major four and two bands from A. japonica were closely related to the phyla Proteobacteria and Bacteroidetes, respectively.