Characterization of microsome-associated tobacco BY-2 centrins.

Characterization of microsome-associated tobacco BY-2 centrins.
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微粒体相关烟草 BY-2 中心蛋白的表征。

DOI:
10.1016/s0171-9335(99)80035-1
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发表时间:
1999
影响因子:
6.6
通讯作者:
A. Lambert
A. Lambert
中科院分区:
生物学3区
文献类型:
--
作者:
V. Stoppin;J. Canaday;A. Lambert

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在大多数真核细胞中,Ca 2+结合蛋白centrin与结构化微管组织中心(MTOC)如中心体相关。在这些细胞中,中心蛋白要么形成中心体相关的收缩纤维,要么参与中心体的生物发生。我们的目的是研究中心蛋白在不含中心体样MTOC的高等植物细胞中的功能。我们已经克隆了两个烟草BY-2中心蛋白的cDNA,我们表明,更高的植物中心蛋白定义一个系统发育组的蛋白质不同于中心体相关的中心蛋白。此外,发现烟草中心蛋白主要与微粒体相关,并且不与γ-微管蛋白(一种已知的MTOC标记物)共定位。虽然在细胞周期中中心蛋白的总体水平没有变化,但在细胞板中在末期显著检测到中心蛋白。我们的研究结果表明,在烟草中,中心蛋白的主要部分是不MTOC相关的,并可能参与细胞质分裂过程中的细胞板的形成。
In most eukaryotic cells, the Ca2+-binding protein centrin is associated with structured microtubule-organizing centers (MTOCs) such as centrosomes. In these cells, centrin either forms centrosome-associated contractile fibers, or is involved in centrosome biogenesis. Our aim was to investigate the functions of centrin in higher plant cells which do not contain centrosome-like MTOCs. We have cloned two tobacco BY-2 centrin cDNAs and we show that higher plant centrins define a phylogenetic group of proteins distinct from centrosome-associated centrins. In addition, tobacco centrins were found primarily associated with microsomes and did not colocalize with γ-tubulin, a known MTOC marker. While the overall level of centrin did not vary during the cell cycle, centrin was prominently detected at the cell plate during telophase. Our results suggest that in tobacco, the major portion of centrin is not MTOC-associated and could be involved in the formation of the cell plate during cytokinesis.
DOI: 10.1006/dbio.1995.1259
发表时间: 1995-09
影响因子: 2.7
作者:
Marie Uzawa;J. Grams;B. Madden;D. Toft;J. Salisbury
通讯作者: Marie Uzawa;J. Grams;B. Madden;D. Toft;J. Salisbury
一种γ-微管蛋白相关蛋白,以细胞周期依赖性方式与高等植物的微管阵列相关。
DOI: 10.1242/jcs.104.4.1217
发表时间: 1993
影响因子: 4
作者:
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通讯作者: Palevitz,BA