Characterization of microsome-associated tobacco BY-2 centrins.
Characterization of microsome-associated tobacco BY-2 centrins.
复制标题
微粒体相关烟草 BY-2 中心蛋白的表征。
DOI:
10.1016/s0171-9335(99)80035-1
复制
发表时间:
1999
影响因子:
6.6
通讯作者:
A. Lambert
中科院分区:
文献类型:
--
作者:
V. Stoppin;J. Canaday;A. Lambert
In most eukaryotic cells, the Ca2+-binding protein centrin is associated with structured microtubule-organizing centers (MTOCs) such as centrosomes. In these cells, centrin either forms centrosome-associated contractile fibers, or is involved in centrosome biogenesis. Our aim was to investigate the functions of centrin in higher plant cells which do not contain centrosome-like MTOCs. We have cloned two tobacco BY-2 centrin cDNAs and we show that higher plant centrins define a phylogenetic group of proteins distinct from centrosome-associated centrins. In addition, tobacco centrins were found primarily associated with microsomes and did not colocalize with γ-tubulin, a known MTOC marker. While the overall level of centrin did not vary during the cell cycle, centrin was prominently detected at the cell plate during telophase. Our results suggest that in tobacco, the major portion of centrin is not MTOC-associated and could be involved in the formation of the cell plate during cytokinesis.
影响因子:
2.7
作者:
Marie Uzawa;J. Grams;B. Madden;D. Toft;J. Salisbury
通讯作者:
Marie Uzawa;J. Grams;B. Madden;D. Toft;J. Salisbury
影响因子:
4
作者:
Liu,B;Marc,J;Joshi,HC;Palevitz,BA
通讯作者:
Palevitz,BA