Cytoskeleton-associated glycoproteins from chicken sympathetic neurons and chicken embryo brain.
Cytoskeleton-associated glycoproteins from chicken sympathetic neurons and chicken embryo brain.
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来自鸡交感神经元和鸡胚脑的细胞骨架相关糖蛋白。
DOI：
10.1111/j.1432-1033.1983.tb07651.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
D. Moss
中科院分区：
文献类型：
--
作者：
D. Moss
从鸡交感神经元纯培养物中获得非离子洗涤剂不溶性组分，并在不连续密度梯度的10%-30%界面进一步纯化。这个部分含有肌动蛋白作为主要成分和大约20个多肽，其中一些是糖基化的。在这个片段中，有两个明显的糖蛋白，分子量分别为130 kDa和90 kDa，已被证明与刀豆蛋白A结合；在培养的神经元中，130-kDa糖蛋白也可以用[3H]氨基葡萄糖和[3H]聚焦标记。当细胞在低离子强度缓冲液中裂解并用肌动蛋白在Sepharose 6B柱的空隙体积中洗脱时，两者都被限制在阶梯蔗糖梯度的一个界面上。用相同的分离方法从10日龄鸡胚脑中获得了相同分子量的糖蛋白。一维肽图显示，来自大脑和交感神经元的含碳水化合物肽即使不完全相同，也非常相似。糖蛋白也存在于坐骨神经中，但在缺乏轴突的圆形神经元或成纤维细胞中不能以洗涤剂不溶的形式检测到。因此，它们可能参与轴突细胞骨架与质膜的连接。
A non-ionic detergent-insoluble fraction was obtained from pure cultures of chicken sympathetic neurons and further purified at the 10%-30% interface of a discontinuous density gradient. This fraction contains actin as its major component and approximately 20 further polypeptides some of which are glycosylated. Two conspicuous glycoproteins in this fraction, of molecular masses 130 kDa and 90 kDa, have been shown to bind to concanavalin A; in cultured neurons the 130-kDa glycoprotein may also be labelled with [3H]glucosamine and [3H]fucose. Both are restricted to one interface of the stepped sucrose gradient when cells are lysed in low ionic strength buffer and eluted with actin in the void volume of a Sepharose 6B column. Glycoproteins of the same molecular weight have been obtained by the same isolation procedure from 10-day-old chicken embryo brains. One-dimensional peptide maps show that the carbohydrate-containing peptides from brain and sympathetic neurons are closely similar if not identical. The glycoproteins are also present in sciatic nerve but cannot be detected in a detergent-insoluble form in rounded neurons - lacking axons - or fibroblasts. They might, therefore, be involved in the linkage of the axonal cytoskeleton to the plasma membrane.
从胚胎脑膜纯化的神经细胞粘附分子的化学表征。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hoffman,S;Sorkin,BC;White,PC;Brackenbury,R;Mailhammer,R;Rutishauser,U;Cunningham,BA;Edelman,GM
通讯作者：
Edelman,GM