Evolution of Eukaryal and Archaeal Pseudouridine Synthase Pus10.
Evolution of Eukaryal and Archaeal Pseudouridine Synthase Pus10.
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DOI：
10.1007/s00239-018-9827-y
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发表时间：
2018-01
影响因子：
3.9
通讯作者：
Geisler M
中科院分区：
文献类型：
--
作者：
Fitzek E;Joardar A;Gupta R;Geisler M
在古细菌中，假尿苷合酶Pus 10将尿苷（U）修饰为tRNA的54和55位。相反，在细菌中没有发现Pus 10，其中在这两个位置的修饰通过TrmA（U 54至m5 U 54）和TruB（U 55至m5 U 55）进行。许多真核生物具有明显的冗余;它们的基因组包含古细菌Pus 10和细菌TrmA和TruB的直系同源物。虽然真核生物Pus 10基因与古细菌Pus 10基因共享一个保守的催化结构域，但由于这两个原因，它们的生物学作用尚不清楚。首先，实验证据表明，人Pus 10参与由肿瘤坏死因子相关凋亡诱导配体诱导的凋亡。人类Pus 10的功能是否在tRNA合成的适当位置或除了tRNA合成之外还未知。其次，Pus 10存在于真菌的早期进化分支（如壶菌属Batrachochytrium）中，但在所有调查的双核真菌（子囊菌纲和担子菌纲）中均不存在。我们对测序的基因组进行了全面分析，发现Pus 10，TrmA和TruB的直系同源物存在于所有调查的动物，植物和原生动物中。这表明共同的真核生物祖先拥有所有这三种基因。接下来，我们检查了116个古细菌和真核生物Pus 10蛋白序列，发现Pus 10在所有调查的基因组中都以单拷贝基因存在，尽管祖先的全基因组复制已经发生。这表明可能存在有害的基因剂量效应。我们的研究结果表明，功能冗余导致基因丢失或新功能在不同的进化谱系。
In archaea, pseudouridine (Ψ) synthase Pus10 modifies uridine (U) to Ψ at positions 54 and 55 of tRNA. In contrast, Pus10 is not found in bacteria, where modifications at those two positions are carried out by TrmA (U54 to m5U54) and TruB (U55 to Ψ55). Many eukaryotes have an apparent redundancy; their genomes contain orthologs of archaeal Pus10 and bacterial TrmA and TruB. Although eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes, their biological roles are not clear for the two reasons. First, experimental evidence suggests that human Pus10 participates in apoptosis induced by the tumor necrosis factor–related apoptosis-inducing ligand. Whether the function of human Pus10 is in place or in addition to of Ψ synthesis in tRNA is unknown. Second, Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). We did a comprehensive analysis of sequenced genomes and found that orthologs of Pus10, TrmA and TruB were present in all the animals, plants and protozoa surveyed. This indicates that the common eukaryotic ancestor possesses all the three genes. Next, we examined 116 archaeal and eukaryotic Pus10 protein sequences to find that Pus10 existed as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications had occurred. This indicates a possible deleterious gene dosage effect. Our results suggest that functional redundancy result in gene loss or neofunctionalization in different evolutionary lineages.
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