Immunomodulatory and radioprotective effects of lignans derived from fresh nutmeg mace (Myristica fragrans) in mammalian splenocytes
Immunomodulatory and radioprotective effects of lignans derived from fresh nutmeg mace (Myristica fragrans) in mammalian splenocytes
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DOI：
10.1016/j.intimp.2008.01.012
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发表时间：
2008-05-01
影响因子：
5.6
通讯作者：
Poduval, T. B.
中科院分区：
文献类型：
--
作者：
Checker, Rahul;Chatterjee, Suchandra;Poduval, T. B.
最近，木脂素存在于水提取物中的新鲜肉豆蔻（肉豆蔻果实的假种皮）被证明具有抗氧化特性，在无细胞系统和保护PUC 18质粒免受辐射诱导的DNA损伤。本报告描述了免疫调节和radiomifying属性的木脂素存在于水提取物中的新鲜肉豆蔻豆蔻在哺乳动物脾细胞。这些macelignans（ML）抑制脾细胞对多克隆T细胞分裂原伴刀豆球蛋白A（Con A）的增殖反应。这种增殖抑制是由于细胞周期停滞在G1期和细胞凋亡的增加，如前G1细胞的增加所示。ML诱导的细胞死亡的活化增加具有剂量依赖性。发现它抑制IL-2和IL-4基因对Con A的应答的转录。IL-2，IL-4和IFN-γ细胞因子的产生显着抑制ML在Con A刺激的淋巴细胞以剂量依赖性的方式。ML以剂量依赖性方式保护脾细胞免受辐射诱导的细胞内ROS产生。ML对淋巴细胞无细胞毒性。相反，它显着抑制辐射诱导的脾细胞DNA损伤的DNA片段减少所示。据我们所知，这是第一份报告显示木脂素在哺乳动物细胞中的抗氧化，辐射保护和免疫调节作用。(c)2008 Elsevier B. V.保留所有权利。
Recently, the lignans present in the aqueous extract of fresh nutmeg mace (aril of the fruit of Myristica fragrans) were shown to possess antioxidant properties in cell free systems and protected PUC18 plasmid against radiation-induced DNA damage. The present report describes the immunomodulatory and radiomodifying properties of lignans present in the aqueous extract of fresh nutmeg mace in mammalian splenocytes. These macelignans (ML) inhibited the proliferation of splenocytes in response to polyclonal Tcell mitogen concanavalin A (Con A). This inhibition of proliferation was due to cell cycle arrest in G1 phase and augmentation of apoptosis as shown by increase in pre G1 cells. The increase in activation induced cell death by ML was dose dependent. It was found to inhibit the transcription of IL-2 and IL-4 genes in response to Con A. The production of IL-2, IL-4 and IFN-gamma cytokines was significantly inhibited by ML in Con A-stimulated lymphocytes in a dose dependent manner. ML protected splenocytes against radiation-induced intracellular ROS production in a dose dependent manner. ML was not cytotoxic towards lymphocytes. On the contrary, it significantly inhibited the radiation-induced DNA damage in splenocytes as indicated by decrease in DNA fragmentation. To our knowledge, this is the first report showing the antioxidant, radioprotective and immunomodulatory effects of lignans in mammalian cells. (c) 2008 Elsevier B.V. All rights reserved.