Using capillary electrophoresis mobility shift assay to study the interaction of CdTe quantum dots with bovine serum albumin
Using capillary electrophoresis mobility shift assay to study the interaction of CdTe quantum dots with bovine serum albumin
复制标题
利用毛细管电泳迁移率变动实验研究CdTe量子点与牛血清白蛋白的相互作用
DOI：
10.1016/j.cclet.2008.04.012
复制
发表时间：
2008-06-01
影响因子：
9.1
通讯作者：
Ren, Ji Cun
中科院分区：
文献类型：
--
作者：
Shao, Li Wen;Dong, Chao Qing;Ren, Ji Cun
本工作首次采用毛细管电泳迁移率变动分析法（CEMSA）研究了量子点与蛋白质的相互作用。在这项研究中，牛血清白蛋白（BSA）和碲化镉量子点作为模型样品。我们观察到BSA很容易吸附在CdTe量子点表面，量子点与BSA形成1：1的化学计量比的复合物。通过CEMSA获得的缔合常数值为2.17 +/- 0.27 x 10 6 mol（-1）L-1（25 ℃）。(C)2008年，吉村任。Elsevier B. V.代表中国化学学会出版。All rights reserved.
In this work, the capillary electrophoresis mobility shift assay (CEMSA) was first adopted to study the interaction of protein with quantum dots (QDs). In this study, bovine serum albumin (BSA) and CdTe QDs were used as model samples. We observed that BSA was facilely adsorbed to CdTe QDs surface, and the QD-BSA complex was formed by a 1:1 stoichiometric ratio. A value of 2.17 +/- 0.27 x 10 6 mol(-1) L-1 (at 25 degrees C) for the association constant was obtained by CEMSA. (C) 2008 Ji Cun Ren. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.