Accumulation of viruslike particles in a yeast mutant lacking a mitochondrial pore protein.
Accumulation of viruslike particles in a yeast mutant lacking a mitochondrial pore protein.
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缺乏线粒体孔蛋白的酵母突变体中病毒样颗粒的积累。
DOI：
10.1128/mcb.9.3.1100-1108.1989
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发表时间：
1989
影响因子：
5.3
通讯作者：
Marshallsay,B
中科院分区：
文献类型：
--
作者：
Dihanich,M;vanTuinen,E;Lambris,JD;Marshallsay,B
在酿酒酵母中，线粒体孔蛋白的缺乏不是致命的，但它会损害一些呼吸功能，从而损害对甘油等非发酵碳源的生长。然而，在经历了一段滞后期后，无孔突变细胞适应了甘油的生长，积累了大量的86千道尔顿(KDa)蛋白质(M.Dihanich，K.sua和G.Schatz，EMBO J.6：723-728,1987)和5千碱基的RNA。免疫金标记法将86 kDa蛋白定位于胞浆组分，尽管在早期的细胞组分中大部分与微粒体组分共编辑。当86 kDa蛋白被鉴定为病毒样颗粒(VLP)的主要外壳蛋白时，这种差异就得到了解决。病毒样颗粒由双链核糖核酸(L-A RNA)编码。通过引入ak10或ak3突变消除原始无孔菌株中的VLP，增加了呼吸缺陷，并延长了其在非发酵碳源上的滞后期。即使在一些含有孔蛋白的野生型菌株中，VLP和呼吸功能的同时丧失也是由于mak10或mak3的引入，这一事实表明VLP与线粒体功能之间存在联系。
The lack of mitochondrial porin is not lethal inSaccharomyces cerevisiae, but it impairs some respiratory functions and, therefore, growth on nonfermentable carbon sources such as glycerol. However, after a lag phase porinless mutant cells adapt to growth on glycerol, accumulating large amounts of an 86-kilodalton (kDa) protein (M. Dihanich, K. Suda, and G. Schatz, EMBO J. 6:723–728, 1987) and of a 5-kilobase RNA. Immunogold labeling localized the 86 kDa-protein exclusively to the cytosol fraction, although most of it cosedimented with the microsome fraction in earlier cell fractionations. This discrepancy was resolved when the 86-kDa protein was identified as the major coat protein in viruslike particles (VLPs) which is encoded by a double-stranded RNA (L-A RNA). Elimination of VLPs in the original porinless strain by introduction of themak10or themak3mutation increased the respiratory defect and prolonged its lag phase on nonfermentable carbon sources. The fact that the simultaneous loss of VLPs and respiratory functions are the introduction ofmak10ormak3occurred even in some porin-containing wild-type strains suggests that there is a link between VLP and mitochondrial functions.