Light-induced resistance of broad bean against Botrytis cinerea
Light-induced resistance of broad bean against Botrytis cinerea
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DOI：
10.1111/j.1439-0434.1998.tb04609.x
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发表时间：
1998-10-01
期刊：
影响因子：
--
通讯作者：
Arase, S
中科院分区：
文献类型：
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作者：
Islam, SZ;Honda, Y;Arase, S
研究了光诱导蚕豆对灰霉病菌的抗性。黄光（590 nm max）和红光（650 nm max）对B附着胞侵染菌丝的形成有显著的抑制作用。在离生和附着蚕豆小叶上的灰霉病。接种前用黄光或红光预处理叶片24 h也能抑制附着胞侵染菌丝的形成。这表明B的附着胞穿透失败。灰霉病在黄光或红光下的光诱导抗性不是由于这些波长（575 nm至700 nm）的光对真菌感染行为的影响，而是由于光诱导蚕豆对13.灰叶B的孢子萌发。灰霉病菌（cinerea），在B的孢子悬浮液滴的上清液中。从接种蚕豆叶片回收的灰霉病菌接种后在红光下保持48小时，显着抑制发芽率为8.2%。黑暗条件下，孢子在侵染液滴中的萌发不受抑制，萌发率为92.3%。另一方面，在没有B孢子的水滴中也观察到这种抗真菌活性。在红光下保持48 h的蚕豆叶片上的灰霉病。然而，抑制活性低于感染液滴。这些结果表明，红光照射蚕豆叶片可激活叶片组织中抗真菌物质的产生，这可能是蚕豆对B诱导抗性的原因。灰叶此外，证明了B的感染增加了叶组织中抗真菌物质的积累。灰叶
This study was designed to investigate the light-induced resistance of broad bean (Vicia faba) against Botrytis cinerea. Yellow (590 nm max) and red (650 nm max) light significantly inhibited the formation of infection hypha from appressoria of B. cinerea on both detached and attached broad bean leaflets. Pretreatment of leaflets with yellow or red light for 24 h before inoculation, also inhibited the formation of infection hypha from appressoria. This indicates that the failure in appressorial penetration of B. cinerea under yellow or red light was not due to the effect of these wavelengths (575 nm to 700 nm) of light on fungal infection behaviour, but due to light-induced resistance in the broad bean against 13. cinerea. Spore germination of B. cinerea, in supernatants of spore suspension droplets of B. cinerea recovered from inoculated broad bean leaflets kept under red light for 48 h after inoculation, was significantly inhibited to a germination rate of 8.2%. However, under darkness the spore germination in infection droplets was not inhibited, with a germination rate of 92.3%. On the other hand, such antifungal activity was also observed in water droplets without spores of B. cinerea on broad bean leaflets kept under red light for 48 h. However, suppressive activity was lower than in infection droplets. These results indicate that irradiation of broad bean leaflets with red light activated production of antifungal substance(s) in the leaf tissues which seem to be responsible for the induced resistance of broad bean against B. cinerea. Further, it was demonstrated that the accumulation of antifungal substance(s) in leaf tissues was increased by infection of B. cinerea.