Clinical and Molecular Epidemiology of Norovirus Infection in Childhood Diarrhea in China
Clinical and Molecular Epidemiology of Norovirus Infection in Childhood Diarrhea in China
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DOI：
10.1002/jmv.22248
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发表时间：
2012-01-01
影响因子：
12.7
通讯作者：
Shu, Xiaoli
中科院分区：
文献类型：
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作者：
Zeng, Mei;Xu, Xiaohua;Shu, Xiaoli
对2008年8月至2009年7月在上海、杭州、重庆和天津的门诊和住院急性非肠道腹泻患儿进行前瞻性调查。采用一步法实时荧光定量RT-PCR检测诺如病毒（Norovirus，NoV）基因组I和II（GI，GII）。基于部分衣壳序列对NoV基因型进行分类。平行检测轮状病毒（RV）。在来自门诊患者的4，123份粪便样本中，1，067份（25.9%）为NoV阳性，其中1，051份（98.5%）属于GII，1，309份（31.7%）为RV阳性。在住院患者组（n = 317）中，25.6%为NoV阳性，41.6%为RV阳性。对1,067株新城疫病毒阳性菌株中的451株进行了测序和基因分型，其中6株为GI型（3株为GI型）。三，二GI。5，1 GI。4)GII型445株。GII菌株聚为9个基因型，包括GII。4 2006年b（69.2%），是唯一的全球创新指数。本研究中发现了4种变体，其次是GII。GII型3例（23.8%）。GII型6例（3.6%）。GII型12例（1.3%）。2例（0.9%），GII. GII型13例（0.4%）。GII型14例（0.2%）。7例（0.2%），GII。16人（0.2%）。在天津的冷季期间观察到NoV感染的高峰，而在上海、杭州和重庆，NoV活性在夏末和秋季之间较高，在冬季较低。NoV是中国儿童腹泻的常见病原体，并且不同地区之间的NoV相关腹泻的季节不同。结果表明，NoV GII. 4 2006 b是2008年至2009年在中国流行的主要菌株。医学病毒学杂志84：145 - 151，2012. (C)2011 Wiley Periodicals，Inc.
A prospective investigation was carried out among pediatric outpatients and inpatients with acute non-dysenteric diarrhea between August, 2008 and July, 2009 in Shanghai, Hangzhou, Chongqing, and Tianjin, China. One step real-time RT-PCR was used for detection of norovirus (NoV) genogroups I and II (GI, GII). The NoV genotypes were classified based on partial capsid sequences. Rotavirus (RV) was detected in parallel. Among 4,123 fecal samples from outpatients, 1,067 (25.9%) were NoV-positive, of which 1,051 (98.5%) belonged to GII and 1,309 (31.7%) were RV-positive. In the inpatient group (n = 317), 25.6% were NoV-positive and 41.6% were RV-positive. Four hundred and fifty-one out of 1,067 NoV-positive strains were sequenced and genotyped and 6 typed strains were GI (3 GI. 3, 2 GI. 5, 1 GI. 4) and 445 typed strains were GII. GII strains clustered into nine genotypes including GII. 4 2006b (69.2%), the only GII. 4 variant identified in this study, followed by GII. 3 (23.8%), GII. 6 (3.6%), GII. 12 (1.3%), GII. 2 (0.9%), GII. 13 (0.4%), GII. 14 (0.2%), GII. 7 (0.2%), and GII. 16 (0.2%). A peak of NoV infections was observed during the cold season in Tianjin, while NoV activity was higher between late summer and autumn and lower during winter in Shanghai, Hangzhou, and Chongqing. NoV is a common causative agent of childhood diarrhea in China and the seasons of NoV-associated diarrhea varies between regions. The results show that NoV GII. 4 2006b was the predominant strain circulating in China between 2008 and 2009. J. Med. Virol. 84:145-151, 2012. (C) 2011 Wiley Periodicals, Inc.