Requirement of the DEAD-box protein Ded1p for messenger RNA translation
Requirement of the DEAD-box protein Ded1p for messenger RNA translation
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DOI：
10.1126/science.275.5305.1468
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发表时间：
1997-03-07
期刊：
影响因子：
56.9
通讯作者：
Chang, TH
中科院分区：
文献类型：
--
作者：
Chuang, RY;Weaver, PL;Chang, TH
DED1基因编码一个假定的RNA解旋酶，在酿酒酵母中参与了核前信使RNA的剪接。遗传和生化分析表明，在两个新分离的ded1条件突变体中，翻译而不是剪接受到严重损害。初步证据表明，翻译的起始步骤可能需要Ded1p蛋白，就像独特的死盒蛋白真核启动因子4A(EIF4A)一样，Ded1基因可以被小鼠同源基因PL10取代，这表明Ded1p在翻译过程中的功能是进化保守的。
The DED1 gene, which encodes a putative RNA helicase, has been implicated in nuclear pre-messenger RNA splicing in the yeast Saccharomyces cerevisiae. It is shown here by genetic and biochemical analysis that translation, rather than splicing, is severely impaired in two newly isolated ded1 conditional mutants, Preliminary evidence suggests that the protein Ded1p may be required for the initiation step of translation, as is the distinct DEAD-box protein, eukaryotic initiation factor 4A (eIF4A), The DED1 gene could be functionally replaced by a mouse homolog, PL10, which suggests that the function of Ded1p in translation is evolutionarily conserved.