Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes
Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes
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DOI：
10.1088/2050-6120/2/2/024007
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发表时间：
2014-06-01
影响因子：
3.2
通讯作者：
Palsson, Lars-Olof
中科院分区：
文献类型：
--
作者：
Carr, Rachel;Puckrin, Robert;Palsson, Lars-Olof
建立了一套圆偏振发光（CPL）光谱仪，并用于研究乳酸盐和四种不同蛋白质与外消旋Eu-Ⅲ和Tb-Ⅲ配合物在水溶液中的结合作用。乳酸结合引起强烈的诱导CPL光谱，所观察到的发射不对称因子与对映体组成呈线性关系。特别强的诱导Tb-III CPL还表征了α-1-酸性糖蛋白的结合相互作用，解离常数K-d为2.5 μ M。
A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic Eu-III and Tb-III complexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, and the observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced Tb-III CPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, K-d, of 2.5 mu M.