EVIDENCE THAT ENDOGENOUS BETA-NERVE GROWTH-FACTOR IS RESPONSIBLE FOR THE COLLATERAL SPROUTING, BUT NOT THE REGENERATION, OF NOCICEPTIVE AXONS IN ADULT-RATS
EVIDENCE THAT ENDOGENOUS BETA-NERVE GROWTH-FACTOR IS RESPONSIBLE FOR THE COLLATERAL SPROUTING, BUT NOT THE REGENERATION, OF NOCICEPTIVE AXONS IN ADULT-RATS
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DOI：
10.1073/pnas.84.18.6596
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发表时间：
1987-09-01
影响因子：
11.1
通讯作者：
VISHEAU, B
中科院分区：
文献类型：
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作者：
DIAMOND, J;COUGHLIN, M;VISHEAU, B
尚未确定β的关键作用。神经生长因子（NGF）在生长反应中的作用，在成年动物的感觉神经元中继续表达。我们现在已经研究了每天给成年大鼠（在一些实验中，小鼠）施用NGF抗血清对（i）未受损的伤害性神经在失神经支配的邻近皮肤中发生的侧支发芽和（ii）皮肤感觉轴突在受损后发生的再生的影响。结果出乎意料。所有侧枝发芽被阻止，已经在进行中的发芽被停止;停止治疗时发芽恢复。相反，重建，甚至扩大，皮肤神经领域再生轴突不受抗神经生长因子治疗，甚至后背根切断术，以消除任何中央营养支持。在失神经支配的皮肤，再生和侧枝发芽轴突利用相同的细胞通路，建立功能相同的领域，从而显示出明显相同的生长行为，但抗神经生长因子治疗清楚地区分它们。我们认为，内源性NFG是负责侧支发芽的伤害性轴突，可能反映了正在进行的功能，神经生长因子在其领域的监管。在成人感觉系统中，NGF在神经生长中的确定作用的这一证明可以应用于成人神经系统中的神经生长因子。然而，伤害性轴突（和非伤害性轴突）的再生不依赖于NGF。
A key role has not yet been identified for .beta. nerve growth factor (NGF) in the growth responses that continue to be expressed in the sensory neurons of adult animals. We have now examined the effects of daily administration to adult rats (and in a few experiments, mice) of antiserum of NGF on (i) the collateral sprouting of undamaged nociceptive nerves that occurs into denervated adjacent skin and (ii) the regeneration of cutaneous sensory axons that occurs after they are damaged. The results were unexpected. All collateral sprouting was prevented and that already in progress was halted; sprouting resumed when treatment was discontinued. In contrast, the reestablishment, and even enlargement, of cutaneous nerve fields by regenerating axons was unaffected by anti-NGF treatment, even after dorsal rhizotomy was done to eliminate any central trophic support. In denervated skin, regenerating and collaterally sprouting axons utilized the same cellular pathways to establish functionally identical fields, thus displaying apparently identical growth behaviors, yet anti-NGF treatment clearly distinguished between them. We suggest that endogenous NFG is responsible for the collateral sprouting of nociceptive axons, probably reflecting an ongoing function of NGF in the regulation of their fields. This demonstration in the adult sensory system of a defined role for NGF in nerve growth could apply in nerve growth factors generally in the adult nervous system. The regeneration, however, of nociceptive axons (and nonnociceptive ones) is not dependent on NGF.