Cortical microtubules contribute to division plane positioning during telophase in maize.
Cortical microtubules contribute to division plane positioning during telophase in maize.
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DOI：
10.1093/plcell/koad033
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发表时间：
2023-04-20
期刊：
影响因子：
11.6
通讯作者：
Rasmussen, Carolyn G.
中科院分区：
文献类型：
--
作者：
Bellinger, Marschal A.;Uyehara, Aimee N.;Allsman, Lindy;Martinez, Pablo;McCarthy, Michael C.;Rasmussen, Carolyn G.
细胞分裂被精确定位以产生正确大小和形状的细胞。在植物细胞中，新的细胞壁是由沿着反平行微管和称为成膜体的微丝阵列运输的小泡在细胞中间建造的。成膜体向细胞皮层的一个特定位置扩展，称为分裂位点，但它如何准确地到达分裂位点尚不清楚。我们观察到微管阵列，积累在细胞皮层的末期过渡期间在玉米（玉米）叶表皮细胞。在成膜体到达细胞皮层之前，这些皮层末期微管与分裂位点短暂地相互作用。当微管接触分裂位点定位的蛋白质TANGLED1或其他密切相关的蛋白质时，微管增加加上末端捕获和暂停发生。微管捕获和暂停使皮质微管在分裂末期垂直于分裂位点。一旦成膜体到达细胞皮层，皮层末期微管主要通过平行成束并入成膜体。微管加入成膜体促进了分裂位点定位的微调。我们的假设是，分裂位点定位的蛋白质，如TANGLED1组织皮质微管在末期介导成膜体定位在最后的分裂平面。微管在末期积累在细胞皮层，并与植物分裂机构相互作用，以指导其向分裂位点运动。
Cell divisions are accurately positioned to generate cells of the correct size and shape. In plant cells, the new cell wall is built in the middle of the cell by vesicles trafficked along an antiparallel microtubule and a microfilament array called the phragmoplast. The phragmoplast expands toward a specific location at the cell cortex called the division site, but how it accurately reaches the division site is unclear. We observed microtubule arrays that accumulate at the cell cortex during the telophase transition in maize (Zea mays) leaf epidermal cells. Before the phragmoplast reaches the cell cortex, these cortical-telophase microtubules transiently interact with the division site. Increased microtubule plus end capture and pausing occur when microtubules contact the division site-localized protein TANGLED1 or other closely associated proteins. Microtubule capture and pausing align the cortical microtubules perpendicular to the division site during telophase. Once the phragmoplast reaches the cell cortex, cortical-telophase microtubules are incorporated into the phragmoplast primarily by parallel bundling. The addition of microtubules into the phragmoplast promotes fine-tuning of the positioning at the division site. Our hypothesis is that division site-localized proteins such as TANGLED1 organize cortical microtubules during telophase to mediate phragmoplast positioning at the final division plane. Microtubules accumulate at the cell cortex during telophase and interact with the plant division machinery to direct its movement towards the division site.
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DOI：
10.1073/pnas.052712299
发表时间：
2002-03-05
影响因子：
11.1
作者：
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通讯作者：
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