Paired Serum and Urine Concentrations of Biomarkers of Diethyl Phthalate, Methyl Paraben, and Triclosan in Rats.
Paired Serum and Urine Concentrations of Biomarkers of Diethyl Phthalate, Methyl Paraben, and Triclosan in Rats.
复制标题
大鼠中邻苯二甲酸二乙酯、对羟基苯甲酸甲酯和三氯生生物标志物的配对血清和尿液浓度。
DOI：
10.1289/ehp.1409586
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发表时间：
2016-01
影响因子：
10.4
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Teitelbaum SL;Li Q;Lambertini L;Belpoggi F;Manservisi F;Falcioni L;Bua L;Silva MJ;Ye X;Calafat AM;Chen J
暴露于环境化学品，包括邻苯二甲酸酯和苯酚，如对羟基苯甲酸酯和三氯生，在美国普通人群中无处不在。这项概念验证啮齿动物研究检查了三种广泛使用的个人护理产品成分[邻苯二甲酸二乙酯（DEP）、对羟基苯甲酸甲酯（MPB）和三氯生]的口服剂量与其各自生物标志物的尿液和血清浓度之间的关系。使用雌性Sprague-Dawley大鼠，我们进行了两轮实验，经口灌胃剂量根据先前研究得出的无明显不良作用水平（NOAEL）选择：1，735（DEP），1，050（MPB），50（三氯生）mg/kg/天。给药剂量范围为0.005 - 173 mg/kg/天，比每种化学品的NOAEL低10- 100，000倍。MPB和三氯生实验的对照是仅用橄榄油（溶剂）处理的动物; DEP血清实验的对照是用最低剂量的MPB和三氯生处理的动物。给药5天，每个给药组5只大鼠。对暴露最后一天采集的尿液和血清进行生物标志物分析。使用线性回归评估口服剂量与生物标志物浓度之间的关系。在所有对照尿样中检测到的生物标志物含量为十亿分之一，表明这三种化学品的地方性环境暴露水平较低，即使采取了所有预防措施也无法控制。在暴露的动物中，所有三种生物标志物的尿液浓度都比血清中的浓度高几个数量级。观察到口服剂量与尿浓度之间始终存在正线性关系（R2 > 0.80）;血清中的这种关系不一致。我们的研究强调了仔细考虑动物实验中使用的口服剂量的重要性，并为未来的研究选择剂量提供了有用的信息。Teitelbaum SL，Li Q，Lambertini L，Belpoggi F，Manservisi F，Falcioni L，Bua L，Silva MJ，Ye X，卡拉法特AM，Chen J. 2016.大鼠中邻苯二甲酸二乙酯、尼泊金甲酯和三氯生生物标志物的配对血清和尿液浓度。环境健康展望124：39-45; http://dx.doi.org/10.1289/ehp.1409586
Exposure to environmental chemicals, including phthalates and phenols such as parabens and triclosan, is ubiquitous within the U.S. general population. This proof-of-concept rodent study examined the relationship between oral doses of three widely used personal care product ingredients [diethyl phthalate (DEP), methyl paraben (MPB), and triclosan] and urine and serum concentrations of their respective biomarkers. Using female Sprague-Dawley rats, we carried out two rounds of experiments with oral gavage doses selected in accordance with no observed adverse effect levels (NOAELs) derived from previous studies: 1,735 (DEP), 1,050 (MPB), 50 (triclosan) mg/kg/day. Administered doses ranged from 0.005 to 173 mg/kg/day, 10–100,000 times below the NOAEL for each chemical. Controls for the MPB and triclosan experiments were animals treated with olive oil (vehicle) only; controls for the DEP serum experiments were animals treated with the lowest doses of MPB and triclosan. Doses were administered for 5 days with five rats in each treatment group. Urine and blood serum, collected on the last day of exposure, were analyzed for biomarkers. Relationships between oral dose and biomarker concentrations were assessed using linear regression. Biomarkers were detected in all control urine samples at parts-per-billion levels, suggesting a low endemic environmental exposure to the three chemicals that could not be controlled even with all of the precautionary measures undertaken. Among the exposed animals, urinary concentrations of all three biomarkers were orders of magnitude higher than those in serum. A consistently positive linear relationship between oral dose and urinary concentration was observed (R2 > 0.80); this relationship was inconsistent in serum. Our study highlights the importance of carefully considering the oral dose used in animal experiments and provides useful information in selecting doses for future studies. Teitelbaum SL, Li Q, Lambertini L, Belpoggi F, Manservisi F, Falcioni L, Bua L, Silva MJ, Ye X, Calafat AM, Chen J. 2016. Paired serum and urine concentrations of biomarkers of diethyl phthalate, methyl paraben, and triclosan in rats. Environ Health Perspect 124:39–45; http://dx.doi.org/10.1289/ehp.1409586