Taenia hydatigena in pigs in Burkina Faso: A cross-sectional abattoir study
Taenia hydatigena in pigs in Burkina Faso: A cross-sectional abattoir study
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DOI：
10.1016/j.vetpar.2016.10.022
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发表时间：
2016-10-30
影响因子：
2.6
通讯作者：
Dorny, Pierre
中科院分区：
文献类型：
--
作者：
Dermauw, Veronique;Ganaba, Rasmane;Dorny, Pierre
棘球绦虫是一种非人畜共患绦虫，以犬为最终宿主，以反刍动物和猪为中间宿主。在猪中，它的存在引起猪带绦虫囊尾蚴病血清学检测的交叉反应。因此，对T.棘球蚴是有效估计T.猪囊虫病在一项横断面屠宰场研究中，我们估计了T。在布基纳法索库杜古屠宰的猪中发现了包虫。研究人员对452头猪的尸体进行了检查，以确定是否存在疑似T。腹腔内或腹腔器官表面的囊尾蚴病变。由当地检验员进行常规肉类检验，以识别T。猪囊尾蚴对所有病变进行PCR-RFLP分析，以区分带绦虫属。此外，使用B158/B60 Ag-ELISA检查个体血液样品中循环囊虫抗原的存在。感知T.在13头猪身上发现了囊尾蚴，而肉类检查人员在7头猪身上发现了感染了T。猪囊尾蚴所有这些都得到了分子分析的证实。在其他疑似病变的猪中，主要位于肝脏，27只和6只被发现携带T。Hydatigena和T.猪囊尾蚴（solium cysticerci）。总的来说，8.8%的猪（40/452）被发现感染了T。棘球蚴感染率为2.9%（13/452）。索利亚在这些阳性猪中，发现1头同时感染两种带绦虫属。(0.2%，1/452）。452头猪中有219头（48.5%）的血清呈阳性反应。确诊为T. Hydatigena和T.血清学检查阳性率分别为57.5%（23/40）和61.5%（8/13）。观察到的T.与非洲的其他研究相比，本研究中的包虫病流行率相对较高。估计活动性猪T。因此，使用B158/1360 Ag-ELISA的猪感染应根据T的存在进行调整。包虫T.在本研究中，在肉类检查中发现的猪感染可能低估了真正的流行率，因为常规肉类检查显示灵敏度较低，而且根据舌头触诊发现感染的猪很少被送往官方屠宰场。(C)2016爱思唯尔B. V.保留所有权利。
Taenia hydatigena is a non-zoonotic cestode that has canines as definitive hosts and ruminants and pigs as intermediate hosts. In pigs, its presence causes cross-reactivity in serological testing for Taenia solium cysticercosis. Therefore, knowledge on the occurrence of T. hydatigena is paramount for validly estimating the seroprevalence of T. solium cysticercosis in pigs. In a cross-sectional abattoir study, we estimated the prevalence of T. hydatigena in pigs slaughtered in Koudougou, Burkina Faso. Carcasses of 452 pigs were examined by investigators for perceived and suspected T. hydatigena cysticercus lesions in the abdominal cavity or on the surface of abdominal organs. Routine meat inspection was performed by local inspectors to identify T. solium cysticerci. All lesions were subjected to PCR-RFLP analysis in order to differentiate Taenia spp. Additionally, individual blood samples were examined for the presence of circulating cysticercus antigens using the B158/B60 Ag-ELISA. Perceived T. hydatigena cysticerci were found in 13 pigs, whereas meat inspectors found seven carcasses infected with T. solium cysticerci. All were confirmed by molecular analysis. Of pigs with other suspected lesions, mostly located in the liver, 27 and six were found to harbour T. hydatigena and T. solium cysticerci, respectively. Overall, 8.8% of pigs (40/452) were found infected with T. hydatigena and 2.9% (13/452) with T. solium. Of these positive pigs, one was found infected with both Taenia spp. (0.2%,1/452). Blood samples of 48.5% of pigs (219/452) were positive in the Ag-ELISA. Pigs with confirmed cysts of T. hydatigena and T. solium had a positive Ag-ELISA result in 57.5% (23/40) and 61.5% (8/13) of cases, respectively. The observed T. hydatigena prevalence in this study is relatively high in comparison to other studies in Africa. Estimates of the occurrence of active porcine T. solium infection using the B158/1360 Ag-ELISA should therefore be adjusted for the presence of T. hydatigena. The low level of T. solium infection detected upon meat inspection in this study is likely an underestimation of the true prevalence since routine meat inspection shows poor sensitivity and pigs perceived to be infected based on tongue palpation are rarely sent to official abattoirs. (C) 2016 Elsevier B.V. All rights reserved.