TISSUE DISTRIBUTION OF THE DEC-205 PROTEIN THAT IS DETECTED BY THE MONOCLONAL-ANTIBODY NLDC-145 .2. EXPRESSION IN-SITU IN LYMPHOID AND NONLYMPHOID TISSUES
TISSUE DISTRIBUTION OF THE DEC-205 PROTEIN THAT IS DETECTED BY THE MONOCLONAL-ANTIBODY NLDC-145 .2. EXPRESSION IN-SITU IN LYMPHOID AND NONLYMPHOID TISSUES
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DOI：
10.1006/cimm.1995.1110
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发表时间：
1995-06-01
影响因子：
4.3
通讯作者：
STEINMAN, RM
中科院分区：
文献类型：
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作者：
WITMERPACK, MD;SWIGGARD, WJ;STEINMAN, RM
大鼠单克隆抗体NLDC-145，已被用作小鼠树突状细胞在许多研究中的标记物，结合的抗原，是更广泛分布，这种抗原是一种独特的205 kDa的膜糖蛋白称为DEC-205，我们最近纯化的数量足以进行基本的生化研究，N-末端测序，和免疫兔。在细胞荧光实验中，新的多克隆抗体和原始的单克隆抗体都检测到DEC-205对许多种类的非树突状小鼠白细胞，特别是B细胞。DEC-205在这些细胞表面上的量比在表皮和骨髓树突状细胞上的量低10至50倍。在这里，我们利用这些试剂，重新评估DEC-205的组织分布的免疫组织化学染色的冷冻切片从各种器官，并通过多器官免疫印迹。DEC-205在胸腺和肠上皮细胞上以及外周淋巴器官的T细胞区域中的树突状细胞上的丰富表达得到组织学证实。此外，DEC-205在其他几个位置可见：B细胞滤泡内的B淋巴细胞、骨髓基质、肺气道上皮细胞和脑毛细血管。免疫印迹证实了从淋巴组织和肺、骨髓和肠制备的裂解物中存在大量DEC-205蛋白。因此，虽然DEC-205在树突状细胞中以高水平表达，但它也在原位由许多其他细胞类型表达。(C)出版社：Academic Press
The rat monoclonal antibody NLDC-145, which has been utilized as a marker for mouse dendritic cells in numerous studies, binds an antigen that is more broadly distributed, This antigen is a unique 205-kDa integral membrane glycoprotein called DEC-205, which we have recently purified in quantities sufficient for basic biochemical studies, N-terminal sequencing, and immunization of rabbits. In cytofluorographic experiments, both the new polyclonal antibody and the original monoclonal detected DEC-205 on many classes of nondendritic murine leukocytes, particularly B cells. The quantities of DEC-205 on the surfaces of these cells were 10 to 50 times lower than those on epidermal and bone marrow dendritic cells. Here we utilize these reagents to reassess the tissue distribution of DEC-205 by immunohistochemical staining of frozen sections from a variety of organs, and by multiple-organ immunoblotting. Abundant expression of DEC-205 was confirmed histologically on thymic and intestinal epithelia and on dendritic cells in the T cell areas of peripheral lymphoid organs. In addition, DEC-205 was visualized in several other locations: B lymphocytes within B cell follicles, the stroma of the bone marrow, the epithelia of pulmonary airways, and the capillaries of the brain. Immunoblotting confirmed the presence of substantial levels of DEC-205 protein in lysates prepared from lymphoid tissues and from lung, marrow, and intestine. Thus, while DEC-205 is expressed at high levels by dendritic cells, it is also expressed by a number of other cell types in situ. (C) 1995 Academic Press, Inc.