Cochlear protection by local insulin-like growth factor-1 application using biodegradable hydrogel
Cochlear protection by local insulin-like growth factor-1 application using biodegradable hydrogel
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DOI：
10.1097/01.mlg.0000200791.77819.eb
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发表时间：
2006-04-01
期刊：
影响因子：
2.6
通讯作者：
Ito, J
中科院分区：
文献类型：
--
作者：
Iwai, K;Nakagawa, T;Ito, J
目的：本实验研究的目的是检查潜在的本地重组人胰岛素样生长因子-1（rhIGF-1）的应用，通过生物可降解的水凝胶治疗耳蜗。研究方法：将浸有rhIGF-1的水凝胶置于Sprague-Dawley大鼠的圆窗膜上，而将浸有生理盐水的水凝胶应用于对照动物。给药后第3天，动物暴露于120 dB声压级（SPL）的白色噪声中2小时。使用听觉脑干反应（ABR）的测量来监测耳蜗功能：频率为8、16和32 kHz。在噪声暴露后7天和30天采集颞骨，定量分析听毛细胞的损失。结果：局部rhIGF-1治疗显著降低噪声暴露后第7天和第30天ABR阈值的升高。组织学分析表明，局部rhIGF-1治疗显着禁止外毛细胞的损失。结论：这些发现表明，通过生物可降解水凝胶局部应用IGF-1具有保护耳蜗免受噪声创伤的潜力。
Objective: The aim of this experimental study was to examine the potential of local recombinant human insulin-like growth factor-1 (rhIGF-1) application through a biodegradable hydrogel for the treatment of cochleae. Methods: A hydrogel immersed with rhIGF-1 was placed on the round window membrane of Sprague-Dawley rats while a hydrogel immersed with physiological saline was applied to control animals. On day 3 after drug application, the animals were exposed to white noise at 120 dB sound pressure level (SPL) for 2 hours. Cochlear function was monitored using measurements of auditory brain stem responses (ABRs) :at frequencies of 8, 16, and 32 kHz. The temporal bones were collected 7 or 30 days after noise exposure and the loss of hair cells was quantitatively analyzed. Results: Local rhIGF-1 treatment significantly reduced the elevation of ABR thresholds on days 7 and 30,after noise exposure. Histologic analysis revealed that local rhIGF-1 treatment significantly prohibited the loss of outer hair cells. Conclusions: These findings demonstrate that local IGF-1 application through the biodegradable hydrogel has the potential for protection of cochleae from noise trauma.