A ninhydrin-based assay to quantitate the total protein content of tissue samples
A ninhydrin-based assay to quantitate the total protein content of tissue samples
复制标题
DOI：
10.1006/abio.2001.5050
复制
发表时间：
2001-05-01
影响因子：
2.9
通讯作者：
Starcher, B
中科院分区：
文献类型：
--
作者：
Starcher, B
对小组织样品的总蛋白含量进行定量对于许多生化分析是必不可少的。在这项研究中，茚三酮法测定总蛋白含量的组织水解物。茚三酮试剂在乙二醇-乙酸钠溶剂系统中可在室温下稳定长达1个月，无需氮气。该反应是非常准确和精确的，与内和测定间的变化小于3%时，5 μ g的蛋白质进行分析。所研究的Ah蛋白质贡献与牛血清白蛋白相同的颜色强度/微克蛋白质。该测定比考马斯反应灵敏数倍，并且在更大的蛋白质浓度范围内呈线性。(C)北京：科学出版社.
Quantitation of small tissue samples for total protein content is essential for many biochemical analyses. In this study a ninhydrin method for measuring the total protein content of tissue hydrolysates is presented. The ninhydrin reagent is stable at room temperature for up to 1 month in the ethylene glycol-sodium acetate solvent system without the requirement for a nitrogen atmosphere. The reaction was very accurate and precise, with intra- and interassay variations of less than 3% when 5 mug of protein was assayed. Ah proteins that were investigated contributed the same color intensity per microgram protein as bovine serum albumin. This assay was several times more sensitive than the Coomassie reaction and linear over a greater range of protein concentration. (C) 2001 Academic Press.