STRUCTURE AND EXPRESSION OF C-ERB-2 AND EGF RECEPTOR GENES IN INFLAMMATORY AND NON-INFLAMMATORY BREAST-CANCER - PROGNOSTIC-SIGNIFICANCE
STRUCTURE AND EXPRESSION OF C-ERB-2 AND EGF RECEPTOR GENES IN INFLAMMATORY AND NON-INFLAMMATORY BREAST-CANCER - PROGNOSTIC-SIGNIFICANCE
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DOI：
10.1002/ijc.2910430205
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发表时间：
1989-02-15
影响因子：
6.4
通讯作者：
RIOU, G
中科院分区：
文献类型：
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作者：
GUERIN, M;GABILLOT, M;RIOU, G
C-erbB-2和表皮生长因子受体（EGFR）基因被独立地证明与乳腺癌进展相关。在这份报告中，我们分析了这两个基因的结构和表达在同一肿瘤标本的一个大系列的乳腺癌。两种临床类型的肿瘤进行了研究：炎症（IBC）和非炎性乳腺癌（NBC）从221未经治疗的患者在不同的临床阶段。c-erbB-2原癌基因的扩增和过度表达分别在27%和47%的肿瘤中观察到，并且与预后最差的乳腺癌，即具有多个阳性腋窝淋巴结的IBC和NBC密切相关。EGRF基因既未扩增也未重排。观察到HindIII内切酶的限制性片段长度多态性（RFLP）。EGFR转录物在46%的肿瘤中被检测到，并且在IBC中比在NBC中更频繁地观察到（p < 0.02）。在NBC中，EGFR转录物的存在与淋巴结受累呈线性增加，并与雌激素受体阴性肿瘤相关（p = 0.01）。对来自相同肿瘤样本的两种基因的分析表明，基因与癌症侵袭性相关。此外，在NBC中这2个基因被独立激活，而在IBC中激活的基因呈负相关，提示c-erbB-2和EGFR基因在NBC和IBC中发挥不同的作用。
C-erbB-2 and epidermal growth factor receptor (EGFR) genes were independently shown to be associated with breast cancer progression. In this report, we have analyzed the structure and expression of these 2 genes in the same tumor specimens of a large series of breast cancers. Two clinical types of tumor were studied: inflammatory (IBC) and non-inflammatory breast cancers (NBC) obtained from 221 untreated patients at different clinical stages. Amplification and over-expression of the c-erbB-2 proto-oncogene were observed in 27% and 47% of tumors, respectively, and were strongly associated with breast cancer of the most unfavorable prognosis, namely IBC and NBC with multiple positive axillary nodes. EGRF gene was neither amplified nor rearranged. A restriction fragment length polymorphism (RFLP) for HindIII endonuclease was observed. EGFR transcripts were detected in 46% of tumors and observed more frequently in IBC than in NBC (p < 0.02). In NBC the presence of EGFR transcripts increased linearly with lymph-node involvement and was associated with estrogen-receptor-negative tumors (p = 0.01). Analysis of both genes from the same tumor samples indicated that genes are associated with cancer aggressiveness. Furthermore, in NBC these 2 genes were independently activated, in contrast in IBC in which activated genes were negatively correlated, suggesting that c-erbB-2 and EGFR genes play different roles in NBC and IBC.