Tricontinental detection of Streptococcus pyogenes M1 UK : A call for wider research and active surveillance
Tricontinental detection of Streptococcus pyogenes M1 UK : A call for wider research and active surveillance
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英国三大洲化脓性链球菌 M1 检测：呼吁更广泛的研究和主动监测
DOI：
10.1101/2023.04.30.538858
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Vieira A
中科院分区：
文献类型：
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作者：
Vieira A
化脓性链球菌M1UK谱系，其特征在于表达SpeA毒素的内在能力，并由核心基因组中的27个单核苷酸多态性定义，占emm1S群体的主导地位。英国的化脓性链球菌该谱系已在欧洲、北美其他地方以及最近的澳大利亚得到确认。然而，SpeA可能不是M1UK谱系成功的唯一决定性因素。属于中间emm1亚系M123SNP的菌株产生SpeA与M1UK菌株没有区别。尽管如此，至少在英格兰，M1UK已经超过了来自M123SNP亚系的菌株。我们推断，健身M1UK驻留在额外的属性，赋予优势的S。化脓菌，强调需要进一步研究。speA上游ssrA前导序列中的单核苷酸多态性（SNP）是区分SpeA生产不同的中间亚系的有限数量的SNP之一。将ssrA SNP引入广泛传播的M1globalclone和中间M113 SNP谱系的代表性分离株（在培养中不能产生容易检测的SpeA）导致SpeA表达，证实了ssrA SNP对SpeA表型的重要性。然而，临床菌株的RNAseq分析表明，SNP的存在并不总是与ssrA前导序列或SpeA表达的通读相关。文献综述表明，通读和speAmRNA转录本长度可能会受到两个组件的调节CovRS的影响，指出细菌染色体和噬菌体编码的超抗原之间的复杂的调控网络相互作用。
TheStreptococcus pyogenesM1UKlineage, characterised by an intrinsic ability to express SpeA toxin and defined by 27 single nucleotide polymorphisms in the core genome, dominates the population ofemm1S. pyogenesisolates in England. The lineage has been identified elsewhere in Europe, North America, and, most recently, Australia. SpeA however may not be the sole deterministic factor underlying success of the M1UKlineage. Production of SpeA by strains belonging to an intermediateemm1 sublineage, M123SNP, is indistinguishable from M1UKstrains. Despite this, in England at least, M1UKhas outcompeted strains from the M123SNPsublineage. We infer that the fitness of M1UKresides in additional properties that confer an advantage toS. pyogenes, underlining a need for further research. A single nucleotide polymorphism (SNP) in the ssrA leader sequence upstream ofspeAis one of a limited number of SNPs that distinguish intermediate sublineages that differ in SpeA production. Introduction of the ssrA SNP into representative isolates of the widely disseminated M1globalclone and the intermediate M113SNPlineage (that cannot otherwise produce readily-detectable SpeA in culture) resulted in SpeA expression, confirming the importance of the ssrA SNP to SpeA phenotype. However, RNAseq analysis of clinical strains showed that presence of the SNP was not invariably linked to read-through from the ssrA leader sequence or SpeA expression. Literature review suggests that read through andspeAmRNA transcript length may be impacted by the two component regulator CovRS, pointing to a complex regulatory network interaction between the bacterial chromosome and phage-encoded superantigens.