Selection for a Zinc-Finger Protein Contributes to Seed Oil Increase during Soybean Domestication
Selection for a Zinc-Finger Protein Contributes to Seed Oil Increase during Soybean Domestication
复制标题
锌指蛋白的选择有助于大豆驯化过程中种子油的增加
DOI：
10.1104/pp.16.01610
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发表时间：
2017-04-01
期刊：
影响因子：
7.4
通讯作者：
Zhang, Jin-Song
中科院分区：
文献类型：
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作者：
Li, Qing-Tian;Lu, Xiang;Zhang, Jin-Song
种子油分是大豆育种驯化的重要农艺性状。虽然已经发现了多个与油脂相关的基因，但目前对种子油脂生物合成的调控机制的了解有限。我们证明，种子的首选基因GmZF 351，编码一个串联CCCH锌指蛋白，在驯化过程中选择。进一步的分析表明，GmZF 351通过直接激活转基因拟南芥（Arabidopsis thaliana）种子中的WRINKLED 1、生物素羧基载体蛋白2、3-酮酰基-酰基载体蛋白合成酶III、二酰基甘油O-酰基转移酶1和油蛋白2来促进油积累。转基因大豆中GmZF 351的过表达也激活了脂质生物合成基因，从而加速了种子油的积累。来自栽培大豆组和野生大豆（Glycine soja）亚组III的ZF 351单倍型与高基因表达水平、种子油含量和启动子活性良好相关，表明选择GmZF 351表达导致栽培大豆中种子油含量增加。我们的研究为种子油脂积累的调控机制提供了新的见解，GmZF 351的操纵可能在提高大豆和其他相关作物的油脂产量方面具有巨大的潜力。
Seed oil is a momentous agronomical trait of soybean (Glycine max) targeted by domestication in breeding. Although multiple oil-related genes have been uncovered, knowledge of the regulatory mechanism of seed oil biosynthesis is currently limited. We demonstrate that the seed-preferred gene GmZF351, encoding a tandem CCCH zinc finger protein, is selected during domestication. Further analysis shows that GmZF351 facilitates oil accumulation by directly activating WRINKLED1, BIOTIN CARBOXYL CARRIER PROTEIN2, 3-KETOACYL-ACYL CARRIER PROTEIN SYNTHASE III, DIACYLGLYCEROL O-ACYLTRANSFERASE1, and OLEOSIN2 in transgenic Arabidopsis (Arabidopsis thaliana) seeds. Overexpression of GmZF351 in transgenic soybean also activates lipid biosynthesis genes, thereby accelerating seed oil accumulation. The ZF351 haplotype from the cultivated soybean group and the wild soybean (Glycine soja) subgroup III correlates well with high gene expression level, seed oil contents and promoter activity, suggesting that selection of GmZF351 expression leads to increased seed oil content in cultivated soybean. Our study provides novel insights into the regulatory mechanism for seed oil accumulation, and the manipulation of GmZF351 may have great potential in the improvement of oil production in soybean and other related crops.