Half-sib progeny evaluation and selection of potatoes resistant to the US8 genotype of Phytophthora infestans from crosses between resistant and susceptible parents
Half-sib progeny evaluation and selection of potatoes resistant to the US8 genotype of Phytophthora infestans from crosses between resistant and susceptible parents
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DOI：
10.1023/a:1015763207980
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发表时间：
2002-01-01
期刊：
影响因子：
1.9
通讯作者：
Kirk, WW
中科院分区：
文献类型：
--
作者：
Bisognin, DA;Douches, DS;Kirk, WW
本研究的目的是评价马铃薯（Solanum tuberosum L.）晚疫病（致病疫霉（蒙特）de Bary）抗性亲本进行品种开发，并鉴定具有中等至高度晚疫病抗性且具有可接受的成熟度和块茎品质的上级无性系。在8个具有晚疫病抗性的不适应亲本（B 0718 -3、Bertita、Bzura、Greta、Libertas、Stobrawa、Tollocan和Zarevo）和适应北美生长条件的易感亲本（栽培品种或高级育种克隆）之间进行95个杂交。在温室和田间使用US 8致病疫霉分离物（A2交配型，甲霜灵抗性）的混合物评估了总共408个田间选择的克隆的晚疫病抗性，所述US 8致病疫霉分离物克服了除R8和R9之外的所有已知R基因。1999年对1998年温室试验中感病叶面积小于或等于10%或1998年田间试验中感病叶面积小于或等于0.30 RAUDPC（相对病害进展曲线下面积）的无性系进行了再测。共选择了118个（占408个的29%）推定的晚疫病抗性克隆。8个抗晚疫病亲本在传递晚疫病抗性的能力和传递给后代的抗性水平上都不同。Tollocan和B 0718 -3家系（半同胞后代）的抗性程度和抗性克隆频率最高。Scott-Knott聚类分析将79个无性系（占118个无性系的67%）归入高抗和中抗晚疫病组。在这79个无性系中，有19个无性系的蔓熟期等于或早于中熟期，且块茎品质合格。在2000年的进一步选择导致了8个先进的选择克隆（6个来自Tollocan和两个来自B 0718 -3家庭）与父母结合葡萄成熟度和块茎质量相当于大西洋，在北美芯片加工的标准品种相同的电阻水平。结果表明，该育种方法可用于晚疫病抗性育种的亲本选择，并可用于筛选抗晚疫病、成熟度和块茎品质优良的上级无性系。
The objectives of this study were to evaluate the use of potato ( Solanum tuberosum L.) late blight (Phytophthora infestans (Mont.) de Bary) resistant parents in cultivar development and identify superior clones possessing moderate to high late blight resistance combined with acceptable maturity and tuber quality. Ninety-five crosses were made between eight unadapted parents with reported late blight resistance (B0718-3, Bertita, Bzura, Greta, Libertas, Stobrawa, Tollocan and Zarevo) and susceptible parents (cultivars or advanced breeding clones) adapted to North American growing conditions. A total of 408 field selected clones were assessed for late blight resistance in the greenhouse and in the field using a mixture of US8 P. infestans isolates (A2 mating type, metalaxyl resistant) that overcame all known R-genes except R8 and R9. Clones with less than or equal to 10% infected foliar area in the greenhouse test or less than or equal to 0.30 RAUDPC (relative area under the disease progress curve) value in the field in 1998 were re-tested in 1999. A total of 118 (29% of 408) putative late blight resistant clones were selected. The eight late blight resistant parents differed in both the ability to transmit late blight resistance and in the level of resistance transmitted to the progeny. The Tollocan and B0718-3 families (half-sib progeny) had the greatest degree of resistance and frequency of resistant clones. Scott-Knott cluster analysis ranked 79 clones (67% of 118) in the high and moderate late blight resistant groups. Among these 79 clones, 19 clones had vine maturity equal to or earlier than mid-season combined with acceptable tuber quality. Further selection in 2000 resulted in eight advanced selected clones (six from Tollocan and two from B0718-3 families) with the same level of resistance as the parent combined with vine maturity and tuber quality equivalent to Atlantic, a standard cultivar for chip processing in North America. The results indicate that this breeding approach can be used to select parents for late blight resistance breeding and to identify superior clones with high levels of late blight resistance and marketable vine maturity and tuber quality.