DEVELOPMENT AND OPTIMIZATION OF A STANDARD METHOD FOR EXTRACTION OF MICROPLASTICS IN MUSSELS BY ENZYME DIGESTION OF SOFT TISSUES
DEVELOPMENT AND OPTIMIZATION OF A STANDARD METHOD FOR EXTRACTION OF MICROPLASTICS IN MUSSELS BY ENZYME DIGESTION OF SOFT TISSUES
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DOI：
10.1002/etc.3608
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发表时间：
2017-04-01
影响因子：
4.1
通讯作者：
Henry, Theodore B.
中科院分区：
文献类型：
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作者：
Catarino, Ana I.;Thompson, Richard;Henry, Theodore B.
作者比较了贻贝软组织的消化程序和微塑料的提取程序。用1M NaOH，35%HNO3，9.6UHB/mL(单位血红蛋白/毫升)的蛋白酶可以达到完全的组织消化，但使用HNO3会对一些微塑料造成不可接受的破坏。添加到贻贝中的微塑料对氢氧化钠和酶消化的回收率相似(93+/-10%)。作者建议使用基于消化效率、微塑性回收和避免腐蚀性化学物质的工业酶。(C)2016年SETAC
The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 +/- 10%) for NaOH and enzyme digestions. The authors recommend use of industrial enzymes based on digestion efficiency, microplastic recovery, and avoidance of caustic chemicals. (C) 2016 SETAC