Patterning and tissue movements in a novel explant preparation of the marginal zone of Xenopus laevis
Patterning and tissue movements in a novel explant preparation of the marginal zone of Xenopus laevis
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DOI：
10.1016/j.modgep.2004.01.001
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发表时间：
2004-07-01
影响因子：
1.2
通讯作者：
DeSimone, D
中科院分区：
文献类型：
--
作者：
Davidson, LA;Keller, R;DeSimone, D
非洲爪蟾胚胎的发育提供了一个适应性强的框架，用于快速评估引导图案形成和形态发生的分子因子。我们提出并表征了一种新的外植体制备，这是有用的，这样的研究。该制备物由在纤连蛋白包被的基质上培养的早期爪蟾原肠胚的180度边缘区组成。除了对其制备过程进行全面描述外，我们还分析了三个发展阶段的基因表达模式。该外植体的定型形态发生可以通过提供表皮、前神经和后神经、脊索、体节中胚层和中内胚层的明确身份的基因表达模式的目录在完整胚胎的背景下理解。(C)2004 Elsevier B.V.保留所有权利。
Development of the Xenopus embryo has provided an adaptable framework for the rapid evaluation of molecular factors that guide patterning and morphogenesis. We present and characterize a novel explant preparation that is useful for such studies. This preparation consists of 180degrees of the marginal zone of the early Xenopus gastrula cultured on a fibronectin-coated substrate. In addition to a thorough description of its preparation, we analyze gene expression patterns at three stages of development. The stereotypic morphogenesis of this explant can be understood in the context of the intact embryo through a catalog of gene expression patterns providing definitive identities for epidermis, anterior and posterior neural, notochord, somitic mesoderm, and mesendoderm. (C) 2004 Elsevier B.V. All rights reserved.