No gene flow between wing forms and clonal reproduction by males in the long-winged form of the ant Vollenhovia emeryi
No gene flow between wing forms and clonal reproduction by males in the long-winged form of the ant Vollenhovia emeryi
复制标题
在长翅蚂蚁 Vollenhovia emeryi 中，翅膀形态和雄性克隆繁殖之间没有基因流动
DOI：
10.1007/s00040-010-0131-0
复制
发表时间：
2011
期刊：
影响因子：
1.3
通讯作者：
K.Ohkawara
中科院分区：
文献类型：
--
作者：
Kobayashi1 K.;E.Hasegawa;K.Ohkawara
短翅蜂后产生的雄蜂（S雄蜂）与长翅蜂后产生的雄蜂（L蜂后）基因组视蛋白基因的基因型相同，而不是同居的S蜂后。这一事实表明，以下两个事件之一可能已经发生，（1）最近的基因流之间的S男性和L皇后或（2）过去的杂交事件之间的。为了验证这些假设，我们分析了L雄虫、L蚁后和L蚁后受精囊精子的核基因组和线粒体DNA。结果表明，L型雄虫和L型蜂王之间以及精子和L型蜂王之间的基因型频率差异显著。精子的线粒体单倍型与它们的女王一致。这些结果表明：（1）L型蜂王只与L型蜂王产生的雄性交配，因此没有从S型雄性到L型蜂王的基因流;（2）L型雄性和S型雄性一样克隆产生儿子;（3）S型雄性和L型蜂王之间的基因型相似性表明过去的杂交事件。
InVollenhovia emeryi, males produced by the short-winged queens (S males) have the same genotype of genomic opsin gene as the long-winged queens (L queens) rather than the cohabiting S queens. This fact suggests that either one of the following two events might have occurred, (1) a recent gene flow between the S males and the L queens or (2) a past hybridization event between them. In order to test these hypotheses, we analyzed the nuclear genome and the mitochondrial DNA of L males, L queens, and sperm from the L queens’ spermathecae. Results showed that genotype frequencies differed significantly between L males and L queens and between the sperm and L queens. Mitochondrial haplotypes of the sperm were consistent with their queen. These indicate that (1) queens of the L form mated only with the males that have been produced by L queens, and thus there is no gene flow from S males to L queens; (2) males of the L-form clonally produce sons as males of the S-form do; and (3) genotype similarity between S males and L queens indicates a past hybridization event.