Effect of surfactant-induced cell surface modifications on electron transport system and catechol 1,2-dioxygenase activities and phenanthrene biodegradation by Citrobacter sp SA01
Effect of surfactant-induced cell surface modifications on electron transport system and catechol 1,2-dioxygenase activities and phenanthrene biodegradation by Citrobacter sp SA01
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表面活性剂诱导的细胞表面修饰对电子传递系统和儿茶酚 1,2-双加氧酶活性以及柠檬酸杆菌 SA01 菲生物降解的影响
DOI：
10.1016/j.biortech.2012.07.059
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发表时间：
2012-11-01
影响因子：
11.4
通讯作者：
Zhu, Lizhong
中科院分区：
文献类型：
--
作者：
Li, Feng;Zhu, Lizhong
为了更好地了解表面活性剂对疏水有机物（HOCs）生物降解的影响，以柠檬酸杆菌（Citrobacter sp.）SA 01为研究对象，研究了表面活性剂吐温80（Tween 80）和十二烷基苯磺酸钠（SDBS）对细胞表面疏水性（CSH）、电子传递系统（ETS）活性和菲生物降解的影响。与未添加表面活性剂相比，添加50 mg/L的表面活性剂Tween 80和SDBS可使CSH、ETS活性、邻苯二酚1，2-双加氧酶（C12）活性分别提高19.8- 25.2%、352.1-376.0 μ mol/g min、50.8-52.7 U/L和8.9-17.2%。在两种表面活性剂存在下，脂多糖（LPS）释放为334.7 μ g/mg，而在它们不存在下，仅释放8.6-44.4 μ g/mg的LPS。因此，增强的LPS释放可能通过增加CSH而增加ETS和O2活性以及菲生物降解。结果表明，表面活性剂增强的CSH提供了一个简单的，但有效的策略，现场应用的表面活性剂增强的生物修复的HOCs。(C)2012爱思唯尔有限公司保留所有权利。
In order to better understand how surfactants affect biodegradation of hydrophobic organic compounds (HOCs), Tween 80 and sodium dodecyl benzene sulfonate (SDBS), were selected to investigate effects on cell surface hydrophobicity (CSH), electron transport system (ETS) activities and phenanthrene biodegradation by Citrobacter sp. SA01. Tween 80 and SDBS increased CSH by 19.8-25.2%, ETS activities by 352.1-376.0 mu mol/g min, catechol 1,2-dioxygenase (C12) activities by 50.8-52.7 U/L, and phenanthrene biodegradation by 8.9-17.2% separately in the presence of 50 mg/L of surfactants as compared to in their absence. Lipopolysaccharide (LPS) release was 334.7 mu g/mg in the presence of both surfactants whereas in their absence only 8.6-44.4 mu g/mg of LPS was released. Thus, enhanced LPS release probably increased ETS and 02 activities as well as phenanthrene biodegradation by increasing CSH. The results demonstrate that surfactant-enhanced CSH provides a simple, yet effective strategy for field applications of surfactant-enhanced bioremediation of HOCs. (C) 2012 Elsevier Ltd. All rights reserved.