Nanomaterial Biointerfacing via Mitochondrial Membrane Coating for Targeted Detoxification and Molecular Detection
Nanomaterial Biointerfacing via Mitochondrial Membrane Coating for Targeted Detoxification and Molecular Detection
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DOI：
10.1021/acs.nanolett.1c00238
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发表时间：
2021-03-09
期刊：
影响因子：
10.8
通讯作者：
Zhang, Liangfang
中科院分区：
文献类型：
--
作者：
Gong, Hua;Zhang, Qiangzhe;Zhang, Liangfang
来源于各种细胞来源的天然细胞膜已成功地用于包覆纳米材料以进行功能化。然而，来自真核生物细胞器的细胞内膜仍然未被探索。在此，我们选择线粒体作为代表性的细胞器和涂层外线粒体膜（OMM）从小鼠肝脏到纳米粒子和场效应晶体管（FET）通过膜囊泡基板融合过程。涂有OMM的聚合物纳米颗粒（OMM-NPs）可以与ABT-263结合，ABT-263是一种靶向OMM的B细胞淋巴瘤蛋白2（Bcl-2）抑制剂。因此，OMMNP在体外有效地保护细胞免于ABT-263诱导的细胞死亡和凋亡，并在体内减弱ABT-263诱导的血小板减少症。同时，用OMM涂覆的FET传感器（OMM-FET）可以检测和区分抗Bcl-2抗体和小分子激动剂。总之，这些结果表明，OMM可以被涂覆到纳米颗粒和功能器件的表面上，这表明细胞内膜可以用作新型生物界面的涂层材料。
Natural cell membranes derived from various cell sources have been successfully utilized to coat nanomaterials for functionalization. However, intracellular membranes from the organelles of eukaryotes remain unexplored. Herein, we choose mitochondrion as a representative cell organelle and coat outer mitochondrial membrane (OMM) from mouse livers onto nanoparticles and field-effect transistors (FETs) through a membrane vesicle-substrate fusion process. Polymeric nanoparticles coated with OMM (OMM-NPs) can bind with ABT-263, a B-cell lymphoma protein 2 (Bcl-2) inhibitor that targets the OMM. As a result, OMMNPs effectively protect the cells from ABT-263 induced cell death and apoptosis in vitro and attenuated ABT-263-induced thrombocytopenia in vivo. Meanwhile, FET sensors coated with OMM (OMM-FETs) can detect and distinguish anti-Bcl-2 antibody and small molecule agonists. Overall, these results show that OMM can be coated onto the surfaces of both nanoparticles and functional devices, suggesting that intracellular membranes can be used as coating materials for novel biointerfacing.