TUMOR-NECROSIS-FACTOR IN SERUM AND SYNOVIAL-FLUID OF PATIENTS WITH ACTIVE AND SEVERE RHEUMATOID-ARTHRITIS
TUMOR-NECROSIS-FACTOR IN SERUM AND SYNOVIAL-FLUID OF PATIENTS WITH ACTIVE AND SEVERE RHEUMATOID-ARTHRITIS
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DOI：
10.1136/ard.49.9.665
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发表时间：
1990-09-01
影响因子：
27.4
通讯作者：
BAGLIONI, C
中科院分区：
文献类型：
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作者：
TETTA, C;CAMUSSI, G;BAGLIONI, C
检查了 15 份类风湿性关节炎 (RA) 患者的血清样本和 29 份滑液样本中是否存在肿瘤坏死因子 (TNF)。 TNF 的测定基于该细胞因子对组织培养中的人黑色素瘤细胞的细胞毒活性。在患有严重RA的患者的血清样本中发现了高浓度的TNF，这些患者的红细胞沉降率和血清α2巨球蛋白增加，但血红蛋白和血清铁浓度降低。 29名患者中有16名的滑液中也发现了肿瘤坏死因子。在白细胞>1010/l 的液体中发现高 TNF 浓度。在正常人的血清或骨关节炎患者的滑液中未检测到肿瘤坏死因子。炎症介质，例如 TNF，可能会导致 RA 的严重程度。
Fifteen serum samples and 29 synovial fluids of patients with rheumatoid arthritis (RA) were examined for the presence of tumour necrosis factor (TNF). The assay of TNF was based on the cytotoxic activity of this cytokine for human melanoma cells in tissue culture. High concentrations of TNF were found in serum samples of patients with severe RA, who had increased erythrocyte sedimentation rate and serum .alpha.2 macroglobulin, but decreased haemoglobin and serum iron concentrations. Tumour necrosis factor was also found in the synovial fluid of 16 out of 29 patients. High TNF concentrations were found in fluids with >1010 leucocytes/l. Tumour necrosis factor was not detected in the serum of normal subjects or in synovial fluid of patients with osteoarthritis. A mediator of inflammation, such as TNF, may contribute to the severity of RA.