The catabolic pathways of in situ rhizosphere PAH degraders and the main factors driving PAH rhizoremediation in oil-contaminated soil
The catabolic pathways of in situ rhizosphere PAH degraders and the main factors driving PAH rhizoremediation in oil-contaminated soil
复制标题
石油污染土壤根际PAH原位降解剂的分解代谢途径及驱动PAH根际修复的主要因素
DOI：
10.1111/1462-2920.15790
复制
发表时间：
2021-10-16
影响因子：
5.1
通讯作者：
Zhang, Gan
中科院分区：
文献类型：
--
作者：
Li, Jibing;Luo, Chunling;Zhang, Gan
根茎修复技术是一种很有潜力的多环芳烃（PAH）修复技术。然而，原位根际多环芳烃降解物的分解代谢途径和驱动多环芳烃根际修复的主要因素尚不清楚。为了解决这些问题，本研究首次采用稳定同位素探测、宏基因组学和分子生态网络分析相结合的方法，研究了三种不同草原牧草对菲的根茎修复作用。所有根际的菲去除率都显著增加，并且通过增加菲降解菌的数量和与其他微生物的相互作用，显著改变了菲降解菌的多样性。在所有的活性菲降解菌中，海洋杆菌科和肠杆菌科分别在裸根际和柳枝稷根际占主导地位；黑麦草和高羊茅根际中无色杆菌显著富集。C-13-DNA宏基因组显示了每个根际中菲降解的几个完整途径，这清楚地解释了它们独特的根修复机制。此外，碳水化合物的丙酸和磷酸肌醇是通过加强土壤微生物群落生态网络来促进多环芳烃根根修复的主导因子。在根际和非根际处理中添加这两种物质的结果证实了这一点，进一步证实了它们在去除多环芳烃和原位修复多环芳烃根际中的关键作用。我们的研究为多环芳烃污染场地的原位根茎修复机制提供了新的见解。
Rhizoremediation is a potential technique for polycyclic aromatic hydrocarbon (PAH) remediation; however, the catabolic pathways of in situ rhizosphere PAH degraders and the main factors driving PAH rhizoremediation remain unclear. To address these issues, stable-isotope-probing coupled with metagenomics and molecular ecological network analyses were first used to investigate the phenanthrene rhizoremediation by three different prairie grasses in this study. All rhizospheres exhibited a significant increase in phenanthrene removal and markedly modified the diversity of phenanthrene degraders by increasing their populations and interactions with other microbes. Of all the active phenanthrene degraders, Marinobacter and Enterobacteriaceae dominated in the bare and switchgrass rhizosphere respectively; Achromobacter was markedly enriched in ryegrass and tall fescue rhizospheres. Metagenomes of C-13-DNA illustrated several complete pathways of phenanthrene degradation for each rhizosphere, which clearly explained their unique rhizoremediation mechanisms. Additionally, propanoate and inositol phosphate of carbohydrates were identified as the dominant factors that drove PAH rhizoremediation by strengthening the ecological networks of soil microbial communities. This was verified by the results of rhizospheric and non-rhizospheric treatments supplemented with these two substances, further confirming their key roles in PAH removal and in situ PAH rhizoremediation. Our study offers novel insights into the mechanisms of in situ rhizoremediation at PAH-contaminated sites.