Apoptosis and G2/M arrest induced by Allium ursinum (ramson) watery extract in an AGS gastric cancer cell line.
Apoptosis and G2/M arrest induced by Allium ursinum (ramson) watery extract in an AGS gastric cancer cell line.
复制标题
AGS胃癌细胞系中葱属（Ramson）水提取物引起的凋亡和G2/M停滞。
DOI：
10.2147/ott.s45865
复制
发表时间：
2013
影响因子：
4
通讯作者：
Jin YN
中科院分区：
文献类型：
--
作者：
Xu XY;Song GQ;Yu YQ;Ma HY;Ma L;Jin YN
本研究旨在确定 Allium ursinum L (ramson) 是否可以抑制人 AGS 胃癌细胞的增殖。此外，我们试图确定这种抑制是否可以通过靶向细胞周期的调节元件来发生。采用流式细胞术观察拉姆森水提取物处理或未处理的AGS细胞系的凋亡和细胞周期。通过蛋白质印迹法检测与细胞周期相关的蛋白质。根据制造商的说明，使用比色测定试剂盒测量半胱天冬酶活性。 Ramson 水提取物诱导 AGS 细胞凋亡和 G2/M 期停滞。 Western blotting显示ramson水提取物对cyclin B有抑制作用。然而，G1期相关蛋白在治疗后保持不变。我们的结果表明，ramson 通过调节细胞周期的要素，有效抑制 AGS 细胞的增殖并诱导细胞凋亡和 G2/M 期停滞。
The present study was designed to determine whether Allium ursinum L (ramson) could inhibit the proliferation of human AGS gastric cancer cells. Furthermore, we attempted to determine whether this inhibition could occur by targeting regulatory elements of the cell cycle. Flow cytometry was used to observe apoptosis and the cell cycle in AGS cell lines treated or not treated with ramson watery extract. Proteins related to the cell cycle were detected by Western blotting. Caspase activity was measured using a colorimetric assay kit according to the manufacturer’s instructions. Ramson watery extract induced apoptosis and G2/M phase arrest in AGS cells. Western blotting showed that cyclin B was inhibited by ramson watery extract. However, G1 phase-related proteins remain unchanged after treatment. Our results indicate that ramson effectively sup pressed proliferation and induced apoptosis and G2/M arrest in AGS cells by regulating elements of the cell cycle.